全 文 :书!"#$%&
,2016,36(6):1105-1109
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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犆犾狅狀犻狀犵犪狀犱犈狓狆狉犲狊狊犻狅狀狅犳犎犎犜犌犲狀犲犻狀‘犎狌犪狀犵犺狌犪’犘犲犪狉犪狀犱
犐狋狊犅狌犱犕狌狋犪狀狋‘犔ü犺狌犪狀犵犺狌犪’犘犲犪狉(犘狔狉狌狊狆狔狉犻犳狅犾犻犪犖犪犽犪犻)
LZhaoqing,RENDandan,ZHOUHe,QIAOYushan
(ColegeofHorticulture,NanjingAgriculturalUniversity,Nanjing210095,China)
犃犫狊狋狉犪犮狋:Thestudyusedfruitpeelofsandpear(犘狔狉狌狊狆狔狉犻犳狅犾犻犪Nakai)cultivars‘Huanghua’pear(rus
setfruit)anditsbudmutant‘Lühuanghua’pear(greenfruit)at8weeksafterfulbloom(WAFB)asex
perimentmaterials.thecDNAfullengthof犎犎犜gene,whichwasnamed犘狆狔犎犎犜 (GenBankaccession
No.KX131155),wasclonedbyconventionalandnestPCRtechniques.Sequenceanalysisshowedthatthe
fullengthofthe犘狆狔犎犎犜orthologconsistedofa1335bpopenreadingframe(ORF)encodingapoly
peptidecontaining444aminoacidresidues.Themolecularweightofdeducedaminoacidswas49.91kD,
withanisoelectricpoint(狆犐)of4.75,whichhadthehighestsimilarity(98%)andclosestrelationship
withthatin犘狔狉狌狊犫狉犲狋狊犮犺狀犲犻犱犲狉犻.RealtimefluorescencequantitativePCR (qRTPCR)analysisdemon
stratedthattheexpressionof犘狆狔犎犎犜genein‘Huanghua’and‘Lühuanghua’pearpeelwerechanging
constantlyduringthekeyperiod(6-9weeks),andsignificantlyhigherin‘Huanghua’pearpeelthanthat
in‘Lühuanghua’.犘狆狔犎犎犜geneinvolvedintheformationofsandpearrusset/greentraits,itsexpres
sionleveldifferencesmayplayaroleintheformationofsandpearskincolor.
犓犲狔狑狅狉犱狊:犘狔狉狌狊狆狔狉犻犳狅犾犻犪Nakai;犎犎犜gene;cloning;bioinformatics;geneexpression
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:
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