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作 者 ：李 博1，詹亚光1*，徐云刚1，曾凡锁1，张桂芹2

期 刊 ：西北植物学报 2012年 11期 页码：2313~2320

关键词：白蜡属；种间杂交；干旱胁迫；光合特性；杂种优势；

Keywords:Fraxinus L., interspecific hybridization, drought stress, photosynthetic physiological characteristics, hybrid vigor,

摘 要 ：以水曲柳与大叶白蜡杂交F₁为试材并对部分花粉进行了高压静电场处理，探讨干旱胁迫对种间杂交F₁生长量、光合特性和叶绿素含量等指标的影响。结果表明：（1）干旱胁迫处理的F₁在净光合速率、气孔导度、蒸腾速率的降低幅度分别比母本（水曲柳自由授粉子代M₇S和M₈S）平均低5.1%、27.55%和12.05%，F₁的叶绿素含量降低幅度比M₇S和M₈S降低幅度小并且复水后F₁叶绿素回复能力也较强。（2）母本效应对F₁表现出明显的影响。（3）高压静电场处理花粉提高了杂交子代对干旱胁迫的适应能力，有效抑制了受胁迫植株的净光合速率、气孔导度和蒸腾速率的降低，但静电场处理F₁在解除胁迫后叶绿素含量及光合恢复能力方面存在差异。（4）杂交组合M₈A_4T和M₇A_2T的抗旱优势比较明显，杂交组合M₈A_2T对抗旱性比较敏感但也具有很强的恢复力。该研究初步明确了水曲柳×大叶白蜡杂交各组合F₁对干旱胁迫的光合特性的生理响应，为干旱半干旱地区选育优良树种奠定了基础。

Abstract:In this paper,Fraxinus mandshurica open-pollinated progeny and hybrids between F.mandshurica and Fraxinus americana ［some of pollens were treated by high voltage electrostatic field (HVEF)］ were selected as the experimental materials.The growth，photosynthetic characteristics and chlorophyll content under drought stress were studied.The results are as follows:(1)The decrease of the F₁ net photosynthetic rate,transpiration rate and stomatal conductance under drought stress were 5.1%,27.55% and 12.05% lower than that of F.mandshurica open-pollinated progeny,respectively.The decrease of F₁ chlorophyll content was lower than that of M₇S and M₈S.The ability of F₁ chlorophyll recovery was also stronger.(2)The F₁ photosynthetic indices were influenced obviously by female effect.(3) F₁ seedlings of pollen treated by HVEF showed higher drought resistance than that of control plants (pollens not be treated ).While net photosynthetic rate,stomatal conductance and transpiration rate were effectively suppressed to decrease by HVEF.However,the photosynthetic physiological characteristics indicated that different hybrids treated by the HVEF on pollen showed difference.(4)M₈A_4T and M₇A_2T enjoyed obvious disadvantage.M₈A_2T showed sensitive drought resistance but also showed very strong resilience.The preliminary research reveals the physiological response of F₁ photosynthetic characteristics under drought stress in order to provide fine tree species for arid and semi-arid areas.

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