---

作 者 ：谭行之,葛 宇,徐文龙,崔崇士,屈淑平*

期 刊 ：西北植物学报 2013年 4期 页码：697~702

关键词：南瓜；AFLP；SSR；遗传图谱；粒宽；QTL定位；

Keywords:pumpkin, AFLP, SSR, genetic map, seed width, QTL mapping,

摘 要 ：以印度南瓜纯系大粒材料‘0515-1’和小粒材料‘0460-1-1’为亲本,获得193个南瓜F₂单株群体,应用AFLP和SSR分子标记技术进行多态性筛选,构建了含84个标记位点的遗传连锁图谱。结果表明,整个图谱包含12个连锁群,全长683.50 cM,标记平均间距为8.13 cM。采用复合区间定位分析,共检测到控制南瓜籽粒宽度的4个数量性状位点（QTL）,分别位于3个连锁群上,各QTL的贡献率在2.87%~29.68%之间。

Abstract:With the aim of molecular marker assisted selection for seed width in pumpkin (Cucurbita maxima),193 progenies of F₂ generation derived from a cross between the large seed variety ‘0515-1’ and the small seed variety ‘0460-1-1’ in the present study were screened using AFLP and SSR molecular markers for their polymorphic sites,and a genetic linkage map containing 84 polymorphic loci was constructed for identifying seed width trait.The genetic linkage map consisted of 12 linkage groups,and was,totally,683.50 cM in length.The average distance between adjacent loci was 8.13 cM.Further analysis using the composite interval mapping method showed that 4 QTL spread over the 3 linkage groups,the percentage of phenotypic variation explained by individual QTL ranged from 2.87% to 29.68%.

---