摘 要 ：采用研磨法首次从新疆药用植物骆驼刺茎和叶等组织中分离得到20株内生菌并从中筛选出一株对作物致病菌的拮抗活性最强的内生菌XJAS AB 11。通过形态学观察， 生理生化特征以及16S rDNA序列分析最终将菌株XJAS AB 11初步鉴定为枯草芽孢杆菌属（Bacillus sp.）。进一步对该拮抗菌株进行扩大培养，对其代谢产物进行初步研究，经硅胶柱色谱及TLC从具有抑菌作用的活性部位分离出活性单体C2和H2 2。紫外光谱、核磁共振氢谱及质谱分析证明化合物C2和H2 2均属于黄酮类化合物。研究表明，骆驼刺植物内生菌中蕴藏着较丰富的抗菌活性物质，是开发天然抗菌药物的潜在资源。
Abstract:Total 20 strains of endophytic bacteria were isolated from different parts of Alhagi pseudalhagi Desv.for the first time by grinding method and 1 strain XJAS AB 11 was screened out from them with the stronggest antagonistic activity.According to the physiological and biochemical characteristics,16S rDNA sequencing analysis,the XJAS AB 11 was identified as Bacillus sp.The antagonistic strain was further amplification cultured and its metabolites was studied initially with column chromatography on silica gel and TLC chromatography.The active monomers C2 and H2 2 were obtained from the antibacterial phase.UV spectra,1H NMR and MS results showed that compound C2 and H2 2 were flavoniods.The molecular formula of these compounds need to be further studied.Our study indicated that A.pseudalhagi Desv.endophytes have plenty of antimicrobial substances as a potential resources of devolopement of natural antimicrobile drugs.