Abstract:Fourty-three isolates of Colletotrichum spp. were isolated from diseased Hevea brasiliensis collected from 4 different counties in Hainan Province, China. The isolates were identified and the sensitivity of these isolates to carbendazim and prochloraz was determined by mycelium growth rate method. The results indicated that 23 isolates were C. gloesporioides and 20 isolates were C. acutatum. The EC50 values of 43 isolates ranged from (0.332 3-7.425 6) and (0.009 1-0.113 3) mg·L-1, respectively, with an average value of (1.714 1±1.684 7) and (0.036 8±0.023 8) mg·L-1, respectively. The average EC50 value of C. acutatum to carbendazim was significantly higher than C. gloesporioides, with an average value of (2.922 7±1.556 3) and (0.663 2±0.194 4) mg·L-1, respectively. There were no significant difference between the average EC50 value of C. acutatum and C. gloesporioides to prochloraz, with an average value of (0.038 3±0.015 2) and (0.035 5±0.020 1) mg·L-1, respectively. There was no significant correlation between the EC50 values of the tested isolates to carbendazim and prochloraz, which indicated that these two fungicides can be alternative use in disease control.