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作 者 ：戴蓬博, 蓝星杰, 张伟卫, 甘 良, 王 阳, 宗兆锋

期 刊 ：植物病理学报 2016年 46卷 2期 页码：273-279

关键词：鉴定；定殖；壮观链霉菌；棉花枯萎菌；生物防治；

Keywords:identification, colonization, Streptomyces spectabilis, Fusarium oxysporum f. sp. vasinfectum, biological control,

摘 要 ：通过生物学特性观察和16S rDNA基因序列分析鉴定菌株SC11为壮观链霉菌(Streptomyces spectabilis)。该菌可在棉花根际土壤以及棉花根部长期定殖,30 d的定殖密度分别为1.38×10⁵ CFU/g和2.95×10³ CFU/g。2011年和2012年的大田防治试验显示,SC11菌粉对棉花枯萎病防效分别为42.51%和36.70%,与对照50%多菌灵(WP)的防效相比无显著差异,施用90~150 d的防效分别显著高于多菌灵(P<0.05)。与清水对照相比,田间施用SC11菌粉棉花倒三叶面积显著提高52.6%,籽棉和皮棉产量分别增加21.9%和23.0%(P<0.05)。研究结果表明,壮观链霉菌SC11是一株对棉花枯萎病具有应用前景的生防菌株。

Abstract:Based on biological property and 16S rDNA sequence anslysis, actinomycetes strain SC11 was identified as Streptomyces spectabilis. Strain SC11 could establish a stable colonization in cotton rhizosphere and cotton root with populations of 1.38×10⁵ CFU/g and 2.95×10³ CFU/g 30 d after sowing, respectively. Field experiments in 2011 and 2012 showed that the powder formula of strain SC11 exhibited control efficacies (CE) of 42.51% and 36.70% against cotton Fusarium wilt, which was as effective as that of the 50% carbendazim (WP). During 90-150 dpi, the CE of the strain SC11 was significantly higher than that of the carbendazim treatment control (P<0.05). The area of the top 3 leaves treated by the SC11 increased by 52.6% compared with the water treated control, and the yields of seed cotton and ginned cotton increased 21.9% and 23.0% (P<0.05), respectively. Results of above research suggest that the strain SC11 has a potential biocontrol ability against cotton Fusarium wilt.

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