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作 者 ：张鲁刚  张明科

期 刊 ：西北植物学报 2008年 7期

关键词：大白菜；orf224；植物表达载体；遗传转化；

Keywords:Chinese cabbage, orf224, plant expression vector, transformation of expressed vector,

摘 要 ：为了创制新的大白菜雄性不育材料,双酶切重组质粒pMD18-T-CMS7311-orf224和表达载体pWR306后,将CMS7311-orf224基因与线性表达载体pWR306进行定向连接,构建了细胞质雄性不育线粒体基因CMS7311-orf224的植物表达载体pWR-CMS7311-orf224,通过酶切和PCR验证pWR-CMS7311-orf224载体构建正确.采用快速冻融法,将表达载体导入农杆菌EHA105,转化大白菜材料06J28,对转化植株的GUS、PCR和RT-PCR检测表明,获得了2个大白菜转基因植株.

Abstract:In order to create a new cytoplasmic male sterile line of Chinese cabbage,the recombinant plasmid and expression vector pWR306 from double-enzyme digestion were linked directionally with the segments of CMS7311-orf224 gene to construct the plant expressio

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