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作 者 ：马秀灵 王丽萍

期 刊 ：西北植物学报 2002年 6期

关键词：cDNA表达文库；表达载体；盐地碱蓬；耐盐性；抗逆性；

Keywords:cDNA expression library, expression vector, Suaeda salsa,

摘 要 ：以盐生植物盐地碱蓬（Suaeda salsa)地上部分为材料，提取植物总RNA，纯化出mRNA，合成cDNA第一链，得到双链cDNA后，连接入植物表达载体，构建cDNA表达文库。该文库重组子约10^6。将该文库转化农杆菌GV3101，可直接用以转化拟南芥。

Abstract:In order to find new genes related to salt stress,we constructed a Suaeda salsa aerial part tissue cDNA expression library.Halophyte S.salsa was used as plant material,the first strands of cDNAs were synthesized from mRNA.Ligated the cDNAs into th

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