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作 者 ：陈雅丽 周咏芝

期 刊 ：西北植物学报 1992年 1期

关键词：爪哇三七；组织培养；植株再生；

Keywords:Gynura aurantiaca, adventitious bud, differentiation, plant regeneration.,

摘 要 ：本文报道了爪哇三七通过组织培养再生植株的方法。爪哇三七的叶片,叶柄或茎段外植体分别接种于6种分化培养基上,均难以直接分化。在附加1mgL~(12),4-D和0.1mgL~(-1)KT的B_5培养基上的叶片形成无色疏松的愈伤组织转接于MS附加1mgL~(-1)BAP和0.1mg L~(-1)NAA培养基上,5天后开始形成绿色球胚状结构,继而形成不定芽或丛生芽。这些不定芽或丛生芽在锈根培养基中迅速长成根系发达的完整植株。此外,本试验比较了不同浓度及不同组合的激素对爪哇三七外植体的脱分化、再分化的影响。并讨论了此项工作对柑桔裂皮病类病毒(简称CEV)的复制及致病机理研究的意义。

Abstract:Methods for plant regeneration in tissue culture of G.aurantiaca were described. When various explants of G.aurantiaca including leaf, petiole or stem segment were respectively cultured on six kinds of differentiation medium, morphogenetic calli were hard

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