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作 者 ：张根发 李凤霞

期 刊 ：西北植物学报 1994年 1期

关键词：单细胞；草木樨状黄芪；细胞培养；

Keywords:monocells, Astragalus melilotoides Pall., cell-suspension, plant-regenerated, PHA.,

摘 要 ：从草木樨状黄芪的子叶和胚轴诱导的愈伤组织中选取结构松脆的颗粒状愈伤组织，用液体培养基Ｓ１２进行悬浮培养建立了悬浮细胞。取换液３天左右生长旺盛的悬浮系材料分离单细胞，用基本培养基Ｓ１２与不同浓度的植物血球凝集素组成的系列培养基ＭＣ１－ＭＣ６进行液体浅层静置暗培养；形成小愈伤组织后，经Ｍ３（ＭＳ＋２，４－Ｄ ２ｍｇ／Ｌ，ＮＡＡ ０．２ｍｇ／Ｌ，ＫＴ １ｍｇ／Ｌ）培养基增殖，ＭＲ２培养基诱导分化出苗，再

Abstract:The calli with a higher capacity of redifferentiation selected from somaclonal calli of As-tragalus melilotoides Pall.were transfered to fluid medium S_(12) ,which had been screened frommany different media and was suitable for suspension culture of this

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