摘 要：利用常规石蜡制片技术，对杜松小孢子发生及雄配子体的发育过程进行观察。杜松的雄球花3月初开始分化。小孢子囊壁一般包括3层细胞： 表皮、中层和绒毡层。造孢细胞在3月中旬形成，3月下旬形成小孢子母细胞，4月上旬开始减数分裂，4月中旬形成四分体，4月下旬小孢子从四分体释放出来。5月上旬开始散粉，成熟花粉粒为单核花粉粒。散粉后，胚珠内的花粉萌发出一短的花粉管后休眠，第2年4月份开始分裂形成管细胞、不育细胞和2个精子细胞。小孢子母细胞发育表现出不同步现象，部分小孢子母细胞在发育过程中退化，在小孢子囊内形成空腔。小孢子囊在小孢子叶基部远轴面和叶腋处都有发生，小孢子叶球为复合小孢子叶球。
Abstract： The microsporogenesis and the development of male gametophyte of Juniperus rigida (stiff leaf juniper) were observed by means of traditional paraffin wax sections. The results were as follows: The male cone of Stiff leaf Juniper differentiated at the beginning of March. The microsporangium wall of J. rigida comprised one epidermis, one middle layers and a tapetum. The sporogenous cell occurred in the middle of March. The microspore mother cell was formed in late March, and the meiosis of microsporocyte began to occur at the beginning of April. Tetrad formed in the middle of April and microspore was released in late April. The mature pollen was in individual cell form and began to shed at the beginning of May. The pollen stayed in dormancy in the ovule after shedding, and the pollen developed the single nucleus pollen divided by mitosis three times and formed one tube nucleus, one sterile cell and two sperm at the beginning of next April. The development of the microspore mother cell was not synchronous, and some microsporocytes degenerated, resulting in formation of a large cavity in the microsporangium. The microsporophylls bare their microsporangia on the abaxial surface and axillae. The microstrobilus was a complex microstrobilus.
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