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Antifeedant Activities and Effects of Tutin and Its Derivatives on Mythimna separate (Lepidoptera: Noctuidae) and Its Physiological Parameters

羟基马桑毒素及其3种衍生物对粘虫的拒食活性与生理指标的影响


为比较羟基马桑毒素(Tutin)及其衍生物(1,2,3)杀虫活性的差异,采用非选择性叶碟法分别测定其对试虫的拒食活性,采用比色法测定这4种化合物在亚致死剂量下(2.0 mg·mL-1)粘虫的酯酶、2类保护酶和可溶性总糖在48 h内的变化趋势。结果表明:在48 h内的拒食活性衍生物2>衍生物3>衍生物1>Tutin; Tutin、衍生物1和3可致粘虫的酯酶活性和可溶性总糖含量略升高,保护酶活性无明显变化; 衍生物2可使试虫的酯酶活性、可溶性总糖含量和SOD活性显著升高。Tutin的2位羟基进行酰化加成改造后能显著提高其拒食活性,衍生物2是最优结构化合物。

To compare differences in insecticidal activities of tutin and its derivatives (1, 2, 3) on Mythimna separate (Walker), the antifeedant activities of these four compounds were analyzed by using the non-choice leaf disk method. The variations of the esterase activity, two protective enzyme activities, and the total soluble sugar content of M. separate treated with sublethal dose (2.0 mg·mL-1) of tutin and its derivatives within 48 h were determined using colorimetric method. After tutin was restructured, the antifeedant activities of three derivatives were significantly increased within 48 h, and their activity ranked as: derivative 2 > 3 > 1 > tutin. Treated with tutin, derivative 1, and derivative 3, the esterase activity and total soluble sugar content of M. separate increased slightly, and the protective enzyme activity did not significantly change. Under the treatment of derivative 2, the esterase activity, total soluble sugar content, and SOD activity of the tested larvae presented pronounced increases. It was concluded that the acylation and addition reaction at 2-hydroxyl of tutin could significantly enhance the antifeedant activity, and as a result, derivative 2 was considered as the best compound in terms of the antifeedant activity in this study.


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