---

作 者 ：陈昌洁 王志贤 刘革

期 刊 ：林业科学 1989年 25卷 4期 页码：317-322

关键词：松毛虫；质型多角体病毒；生物测定；

Keywords:Pine caterpillar, Cytoplasmic Polyhedrasis Virus, Bioassay,

摘 要 ：应用日本赤松毛虫质型多角体病毒(以下简称JDS-CPV)对马尾松毛虫第一代3龄和5龄幼虫进行了生物测定。结果表明,JDS-CPV对3龄和5龄幼虫的LC₍₅₀₎分别为1.6×10⁵CPB/ml和3.3×10⁵CPB/ml。其回归直线方程分别为Y=2.44+0.49x和Y=1.83+0.57x。对一代3龄虫的LD₍₅₀₎为2.1×10³CPB/ml,回归直线方程为Y=1.72+0.98x。以1×10⁸CPB/ml浓度为例的LT₍₅₀₎,3龄虫为8.5天,5龄虫为13天。证实JDS-CPV对我国马尾松毛虫有较好的毒力。

Abstract:Bioassay of JDS-CPV was conducted on the 3rd instar and 5th instar larvae of pine caterpillar D. punctatus. The regression equations between concentration and mortality for 3rd instar and 5th instar were Y=2.44+0.49x and Y=1.83+0.57x, LC₍₅₀₎ value were 1.6×10⁵PIB/mL and 3.3×10⁵PIB/ml respectively. The regression equation between the count of PIB and mortality for 3rd instar larvae is Y=1.72×0.98x, LD₍₅₀₎ value is 2.1×10³PIB/larvae. For example as 1×10⁸PIB/ml, the LT₍₅₀₎ value for 3rd instar and 5th instar larvae were 8.5 days and 13 days respectively. The results showed that the JDS-CPV has higher virulence to pine caterpillar.

---