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作 者 ：吴克贤 李伟 徐妙珍 党常顺 刘越

期 刊 ：林业科学 1996年 32卷 2期 页码：125-133

关键词：长由落叶松；组织培养；分化；生根；

Keywords:Larix olgensis, Tissue culture, Differentiation, Rooting,

摘 要 ：以长白落叶松叶芽作外值体进行离体培养,经过透导分化和生根,培育出了可用于造林的幼苗。透导芽分化以Z培养基和SH培养基较好,激素组合为BA1.0(单位:mg/L,下同)+HAA0.1,分化率平均55%,最高可达68%左右。诱导再生植株生根以z+IBA0.2+NAA0.2+间苯三酚40效果较好。试验发现,基本培养基是影响生根最重要的因素之一。对供体植株进行一定的前处理可使外植体的分化率提高25.8%。研究认为,落叶松组培繁殖的较好程序应是:选择优良个体→植株的前处理→取材接种→诱导芽分化→继代增殖→诱导生根→移栽。

Abstract:Regenerated plantlets used for forestation were induced from axillary buds culture in vitro of plus trees 5-35 years old of Larix olgensis Henry.Zand SH media supplemented with BA1.0 (mg/L,the following units are the same) and NAA0.1were better for induction of bud differentiation, induction rate of 55% in average and 68% at the most were obtained.Z mediumadded with IBA0.2,NAA0.2and Phloroglucinol 40 was better for rooting.It was observed that the hasal medium takes notable effect on rooting in this experiment.The differentiation rate of explant would be increased by 25.8% with pretreatment for donor plants. Better procedure of micropropagation for larch should be as follows:selecting plus trees→pretreatment of donor plants→inoculation→inducing differentiation of bud→subculture→rooting→transplanting.

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