Abstract:A cytop lasmic male sterile line of Chinese cabbage and two male fertile lines of LeafMustard were app lied to transfer the male sterility. Interspecific crosses followed by 7 generations’ backcrosses withLeafMustards as recurrent parentswere carried out. Two new CMS lines of LeafMustards were obtained. It was identified that the CMS lines of leaf mustard possessed equal number of chromosomes with the recurrent parents, and theirmale sterility were shown as 100% both in the degree and ratio. The morphological traits and the seed set ability of the CMS lines were inclined to the recurrent parents. Four favorite combinations with the CMS as female parentwere got through p lot trail.