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作 者 ：盛艳萍;杨建平;张松;齐宪磊;李新成

期 刊 ：园艺学报 2005年 32卷 02期 页码：318-320

关键词：大葱；成熟胚；愈伤组织诱导；分化；内源激素；

Keywords:Allium fistulosum L., Mature embryos, Callus induction, Differentiation, Endogenous hormones,

摘 要 ：对大葱成熟胚的愈伤组织诱导及分化过程进行了观察, 并对再生过程中内源激素水平进行了测定。研究结果表明: 大葱愈伤组织诱导过程中, ZR在3 d时含量达峰值, 可能是导致6 d时增长率最高的主要原因, ABA在此过程中可能为负调控因子。GA₃ 在愈伤组织分化过程中的含量明显高于继代过程,说明其对大葱愈伤组织的分化有重要作用。适当高的6-BA与NAA比值有利于芽的分化, 但是细胞分裂素类浓度过高时则会对不定芽造成伤害。

Abstract:The callus induction and p lant regeneration from Allium fistulosum L. mature embryos culture were studied in A. f istulosum L. and the endogenous hormone changes in this course were analyzed. The callus growth rate reached the highest point at the 6 th day after culturing, which might be caused by the maximum concentration of ZR at the 3 rd day. ABA was a negative regulation factor in the callus induction. The concentration of GA₃ during callus induction wasmuch higher than that in the multip lication course, which indicated GA₃ was very important in the differentiation period. The appropriate proportion between 6-BA and NAA was beneficial to the shoot differentiation of A. fistulosum L. , but there was side effect to shoot regeneration if CTK concentration was too high.

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