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作 者 ：牛爱国;张开春;张晓明;闫国华;李文生;姜立杰

期 刊 ：园艺学报 2005年 32卷 02期 页码：298-300

关键词：樱桃；种质；试管保存；

Keywords:Cherry, Germplasm, In vitro preservation,

摘 要 ：采用组织培养方法, 以樱桃7个品种试管苗为试材, 研究了樱桃离体保存方法。结果表明, 在室温光培养条件下, 培养基中添加甘露醇10 g·L^-1 , 试管苗可保存6个月; 在低温暗培养条件下, 试管苗生长明显受到抑制, 保存期可延长到14个月; 低温暗培养结合培养基添加甘露醇10 g·L ^-1 , 能使保存期延长至16个月, 继代后成活率达52%～98%。部分品种可延长至20个月, ‘对樱’和‘Gisela 5’继代后成活率分别为45%和58%。

Abstract:An in vitro preservation method of cherry germp lasm was established. Seven varieties from different specieswere used asmaterials. Shootswith two buds cut from in vitro subculture plantlets were planted in F14 medium supplemented with 10 g·L ^{- 1} mannitol, then cultured either at room temperature with illumination, or at 5℃ in darkness. Survival rate, regeneration rate, average stem length and propagation ratio of reactivated cultures after preservation under different temperature and illumination treatmentwere investigated.
The result showed that shoots could survive for six months under room temperature with illumination, and for 16 months at 5℃ in darkness. The survival rate of reactivated cultures after p reservation for 16 mouths at 5℃
in darkness are 52% to 98%. Some varieties could survive for 20 months at 5℃ in darkness, the survival rate of‘Duiying’and ‘Gisela 5’are 45% and 58%.

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