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Comprehensive Evaluation of Germplasm Resource of Elymus nutans in Alpine Region

高寒地区垂穗披碱草种质资源综合评价



全 文 :!23" !6#
 Vol.23  No.6
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Authorforcorrespondence,Email:fuhua@lzu.edu.cn
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:S326    [\]^_:A     [`aZ:10070435(2015)06123908
犆狅犿狆狉犲犺犲狀狊犻狏犲犈狏犪犾狌犪狋犻狅狀狅犳犌犲狉犿狆犾犪狊犿犚犲狊狅狌狉犮犲
狅犳犈犾狔犿狌狊狀狌狋犪狀狊犻狀犃犾狆犻狀犲犚犲犵犻狅狀
ZHANGDianye,ZHANGLijing,HUANGDejun,FUHua
(StateKeyLaboratoryofGrasslandAgroecosystem,ColegeofPastoralAgricultureScienceandTechnology,
LanzhouUniversity,Lanzhou,GansuProvince730020,China)
犃犫狊狋狉犪犮狋:Inordertoselecttheelitegermplasmresourceasthebreedingmaterialsofthecultivarswith
highyieldandgoodqualityinthefuture,thenineyieldandqualitycharactersof45wild犈犾狔犿狌狊狀狌狋犪狀狊
populationswerecomprehensivelyevaluatedbyprincipalcomponentanalysisandclusteranalysis.There
sultsindicatedthateachcharacterof45犈犾狔犿狌狊狀狌狋犪狀狊germplasmresourcehadsignificantdifference.Hey
yieldhadthehighestvariablecoefficient(40.86%),whereasbothneutraldetergentfiberandaciddeter
gentfiberhadlowervariablecoefficients(2.43%and5.37%).Correlationanalysisindicatedthatthere
werevaryingdegreesofconnectionbetweeneachcharacteroftestedmaterials,especialyyieldwassignifi
cantlynegativecorrelationwithwatersolublecarbohydrate,andcrudeproteinwassignificantlynegative
correlationwithagronomiccharacterssuchasplantheightanddrymattercontent,respectively.Thenine
charactersweretransformedtofourprincipalcomponentsthatbasicalyrepresentedthemaininformation
oftheoriginaltargetandthecumulativecontributionratewas85.53%.Clusteranalysisshowedthat45
犈犾狔犿狌狊狀狌狋犪狀狊populationsweredividedinto8categories.Thematerialswouldnotbedividedintothe
samegroupstrictlyaccordingtothesameprovenance,however,theywouldbeclusteredintoonegroupif
theycamefromthesimilarhabitat.Theresultsofprincipalcomponentanalysisandclusteranalysisindica
tedthatthematerialofP16hadthecharacterofhighyield,thematerialsofP22,P23andP42hadthe
characterofhighsugar,thematerialofP36hadthecharacterofhighprotein,andthematerialsofP30,
P31,P38andP39hadbettercomprehensiveperformance.
犓犲狔狑狅狉犱狊:犈犾狔犿狌狊狀狌狋犪狀狊;Germplasmresource;Production;Quality;Comprehensiveevaluation
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Table1 Basicsituationof45犈犾狔犿狌狊狀狌狋犪狀狊materials
WX
Identity
©X?R
Locality

Altitude
/m
RÐE
LongitudeE
/°
$¸t
Annualmean
temperature/℃
$¸ÎŒº
Annualmean
precipitation/mm
P01 ¥¦)Âþ Xiahe,Gansu 3094 102.63 3.07 530.74
P02 ¥¦°’­ Hezuo,Gansu 3111 102.95 4.10 536.28
P03 ¥¦°’­ Hezuo,Gansu 3228 103.02 4.16 541.12
P04 ¥¦°’­ Hezuo,Gansu 3000 102.92 4.39 514.24
P05 ¥¦°’­ Hezuo,Gansu 2923 102.85 4.46 494.74
P06 ¥¦)Âþ Xiahe,Gansu 2977 102.84 3.72 537.39
P07 ¥¦)Âþ Xiahe,Gansu 3000 102.39 2.40 563.30
P08 ¥¦)Âþ Xiahe,Gansu 3264 102.52 3.71 459.47
P09 ¥¦)Âþ Xiahe,Gansu 3194 102.51 3.80 455.07
P10 ¥¦)Âþ Xiahe,Gansu 3066 102.49 3.82 448.43
P11 ¥¦)Âþ Xiahe,Gansu 3515 102.13 2.48 455.40
P12 ¥¦)Âþ Xiahe,Gansu 3017 102.70 3.26 537.96
P13 ¥¦ª§þLuqu,Gansu 3150 102.55 2.74 551.24
P14 ¥¦ª§þLuqu,Gansu 3030 102.66 3.01 579.90
P15 ¥¦ª§þLuqu,Gansu 3050 102.69 3.15 591.28
P16 ¥¦ª§þLuqu,Gansu 3307 102.41 2.48 533.08
P17 ¥¦ª§þLuqu,Gansu 3322 102.64 2.95 629.07
P18 ¥¦ª§þLuqu,Gansu 3490 102.34 2.09 594.96
P19 ¥¦ª§þLuqu,Gansu 3494 102.30 2.27 554.36
P20 ¥¦ª§þLuqu,Gansu 3485 102.53 2.50 605.90
P21 ¥¦« þ Diebu,Gansu 3153 102.72 3.40 630.43
P22 ¥¦o¬þLintan,Gansu 2915 103.33 4.15 569.90
P23 ¥¦o¬þLintan,Gansu 2820 103.41 4.42 590.35
P24 ¥¦o¬þLintan,Gansu 2785 103.50 4.88 587.11
P25 "™èß&þ Ruoergai,Sichuan 3217 102.72 3.43 635.09
P26 "™èß&þ Ruoergai,Sichuan 3173 102.75 3.51 631.22
P27 "™èß&þ Ruoergai,Sichuan 3284 102.57 2.65 610.74
P28 ¥¦ý§þ Maqu,Gansu 3864 102.17 1.56 598.85
P29 ¥¦ý§þ Maqu,Gansu 3710 102.15 1.58 596.12
P30 ¥¦ý§þ Maqu,Gansu 3518 102.07 1.45 602.46
P31 ¥¦ý§þ Maqu,Gansu 3475 102.11 1.50 604.74
P32 ¥¦ý§þ Maqu,Gansu 3518 101.94 1.46 633.19
P33 ¥¦ý§þ Maqu,Gansu 3432 101.94 1.45 588.58
P34 233­þ Haiyan,Qinghai 3272 100.57 1.47 336.93
P35 23Q¬þ Gangcha.Qinghai 3320 100.22 1.11 339.03
P36 23Q¬þ Gangcha.Qinghai 3159 100.21 1.11 338.98
P37 23Q¬þ Gangcha.Qinghai 3195 99.75 1.89 330.70
P38 235eþ Gonghe,Qinghai 3253 99.53 2.80 322.14
P39 235eþ Gonghe,Qinghai 3253 99.53 2.80 322.14
P40 235eþ Gonghe,Qinghai 3248 99.54 2.79 322.14
P41 235eþ Gonghe,Qinghai 3195 100.86 3.30 315.53
P42 235eþ Gonghe,Qinghai 3195 100.86 3.30 315.53
P43 23Q¬þ Gangcha,Qinghai 3301 100.14 1.11 333.42
P44 ¥¦™ÖþZhuoni,Gansu 2536 103.53 5.03 597.65
P45 ¥¦™ÖþZhuoni,Gansu 2847 103.29 4.15 566.62
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Table2 Statisticalanalysesofyieldandqualitycharactersof犈犾狔犿狌狊狀狌狋犪狀狊materials
~~
Characters
ߝI
Minimum
ß(I
Maximum
ã¸I
Mean
éa
Range
:;a
SD
kêI×
犆犞/%
p·ù犉I
犉amongpopulations
%`ݺDP/kg·hm-2 529.43 43792.28 2006.77 3849.84 820.03 40.86 1.942
B^ H/cm 54.20 89.33 68.35 35.13 7.96 11.64 3.626
Gק×RS/No·m-2 1.38 13.89 7.54 12.51 2.80 37.20 2.043
%òpºDMC/% 21.87 29.61 25.35 7.74 1.59 6.27 4.199
s‘~„ WSC/% 1.59 6.03 3.49 4.44 0.92 26.23 5.603
qòCP/% 13.88 23.92 16.99 10.04 1.91 11.23 2.260
ëTP/% 0.17 0.29 0.22 0.13 0.03 12.47 4.460
=~ •uvNDF/% 57.34 64.97 62.23 7.63 1.51 2.43 1.961
/~ •uvADF/% 35.06 47.23 39.64 12.17 2.13 5.37 3.648
  Ô:È60.05ŒãÍ!"M=; È60.01ŒãÍ!"M=,ͤ
Note:meanssignificantcorrelationatthe0.05level;meanssignificantcorrelationatthe0.01level,thesameasbelow
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Table3 Correlationanalysesofyieldandqualitycharactersof犈犾狔犿狌狊狀狌狋犪狀狊materials
~~
Characters
%`ݺ
DP
B^

Gק×
RS
%òpº
DMC
s‘~„
WSC
qò
CP
ë
TP
=~ •uv
NDF
/~ •uv
ADF
%`ݺDP 1
B^ H 0.694 1
Gק×RS 0.865 0.495 1
%òpºDMC -0.416 -0.011 -0.500 1
s‘~„ WSC -0.489 -0.196 -0.516 0.541 1
qòCP -0.040 -0.251 -0.093 -0.374 -0.146 1
ëTP 0.096 -0.141 0.127 -0.475 -0.281 0.396 1
=~ •uvNDF 0.429 0.349 0.507 -0.038 -0.368 -0.604 -0.129 1
/~ •uvADF 0.374 0.414 0.322 -0.005 -0.187 -0.395 -0.078 0.566 1
2421
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Table4 Principalcomponentsandeigenvaluesofagronomictrait
~~
Characters
!1Š7x
ThefirstPC
!2Š7x
ThesecondPC
!3Š7x
ThethirdPC
!4Š7x
ThefourthPC
%`ݺDP 0.90 0.01 0.36 -0.07
B^ H 0.52 -0.47 0.62 0.22
Gק×RS 0.88 0.16 0.01 -0.36
%òpºDMC -0.47 -0.72 0.09 0.24
s‘~„ WSC -0.70 -0.31 0.39 0.02
qòCP 0.04 0.86 0.30 0.16
ëTP 0.10 0.73 -0.17 0.82
=~ •uvNDF -0.56 0.55 0.50 0.07
/~ •uvADF -0.45 0.59 0.11 -0.76
#$IEigenvalue 3.10 2.79 1.05 0.66
y²Contributionrate/% 34.44 31.01 11.72 7.36
—–y²Cumulativecontributionrate/% 34.44 65.45 77.17 84.53
  45ºâãäå`Jò½)Š7x%܄x
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Table5 Factorscoresof45犈犾狔犿狌狊狀狌狋犪狀狊materials
WX
Identity
%܄x
Factorscore
1 2 3 4
l°„x
Synthesis
score
WX
Identity
%܄xFactorscore
1 2 3 4
l°„x
Synthesis
score
P1 -1.58 0.11 0.50 0.41 -0.42 P24 -0.43 -1.92 1.37 -0.58 -0.62
P2 0.75 -2.35 -0.39 0.43 -0.48 P25 -0.57 -2.13 0.14 -0.74 -0.89
P3 -1.71 -0.71 -0.27 1.98 -0.69 P26 0.26 -0.73 2.13 -0.65 0.07
P4 0.23 0.03 -0.33 -0.34 0.02 P27 0.93 -0.19 -0.35 -0.23 0.20
P5 2.21 -0.67 1.05 -0.65 0.63 P28 0.07 -1.16 -0.14 -1.31 -0.45
P6 1.98 -0.31 0.99 -0.07 0.70 P29 0.50 0.44 -0.36 -0.74 0.21
P7 -0.01 0.11 -0.10 1.74 0.15 P30 1.64 3.49 -0.14 1.32 1.73
P8 -0.76 -0.08 -0.06 0.42 -0.26 P31 2.42 2.84 -0.25 -1.77 1.56
P9 0.53 -0.58 -0.18 -0.99 -0.09 P32 1.36 -0.52 -1.74 1.55 0.22
P10 -0.14 -1.88 1.00 0.17 -0.50 P33 1.04 -2.17 0.94 -1.31 -0.30
P11 1.90 -1.05 -0.56 -0.45 0.23 P34 1.57 0.70 -1.11 -1.56 0.51
P12 -0.34 0.91 -0.74 -0.23 0.06 P35 0.50 -0.17 0.10 -1.02 0.06
P13 0.37 0.97 -0.60 0.61 0.40 P36 -2.30 5.38 1.46 1.30 1.14
P14 0.07 -0.21 0.60 1.82 0.16 P37 -3.11 0.85 -0.04 0.85 -0.75
P15 -0.08 0.17 -0.39 1.91 0.12 P38 2.40 3.08 1.91 1.05 2.08
P16 4.39 -1.70 1.58 3.69 1.44 P39 2.38 1.81 0.02 -1.07 1.30
P17 -2.82 0.05 0.15 -0.51 -0.98 P40 -1.81 0.74 0.64 -1.50 -0.43
P18 0.67 -3.91 0.25 -0.50 -0.99 P41 0.87 0.23 -2.17 0.11 0.13
P19 -0.36 -0.20 -0.96 -0.71 -0.35 P42 -3.43 -0.62 0.65 -0.40 -1.33
P20 -2.24 -0.16 -0.07 0.01 -0.83 P43 -2.11 0.53 -2.56 1.49 -0.75
P21 1.66 -0.20 -2.58 -0.16 0.19 P44 0.29 2.12 -0.42 -2.26 0.54
P22 -3.38 -0.82 0.49 0.76 -1.31 P45 -1.36 1.59 0.94 -2.06 -0.02
P23 -2.47 -1.73 -0.38 0.22 -1.42
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)x¾81。!Ⅰ1·s17º½)67,!Ⅱ1·
s7º½)67,!Ⅲ1·s3º½)67,!Ⅳ1
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!Ⅴ、Ⅶ、Ⅷ1·Ñðs1º½)X7ÓI。&1x
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v
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P31,P36,P38,P39eP4259ºJò½)。I9
ºJò=P16!òŠ7xe!"Š7xvIß(,
P22,P23eP42!òŠ7x‘Iߝ,P36!ÁŠ
7xvIß(
,P38!•Š7xvIß^,P30,P31
eP39!òŠ7x¶!ÁŠ7xvId^,¢ðl
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,P38ðݺeþòèqß|l½),Èaß
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,P22,
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1

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ºÈazêÌFX&”+ò1

Æ°Æ%wˆax
£
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P42s‘~„pºd^,P30,P31,P38eP39”Ý
º~~‚þò~~ŠÙd|lÈa
,P36qe
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%FŠ7xx
£%±Sl9º½)=P16s˜’¾^݊§,
P22,P23eP42s˜’¾^„Š§,P36s˜’¾
^Š§ÖËÍò‰lZÔ±J¿’

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‰lÆB±±˜%±zˆBI¥7_þJ

W1 ÓÔÕÖ¤bcÇ«ÝYX„
Fig.1 Hierarchicalclusteranalysisof
犈犾狔犿狌狊狀狌狋犪狀狊materials
4421
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Table6 Averagevaluesof9charactersinthe8groupsof犈犾狔犿狌狊狀狌狋犪狀狊materials
~~
Characters
1· Groups
Ⅰ Ⅱ Ⅲ Ⅳ Ⅴ Ⅵ Ⅶ Ⅷ
%`ݺDP/kg·hm-2 1995.01 2196.75 3200.59 1140.38 773.97 3070.58 1430.77 4379.28
B^ H/cm 65.19 77.88 78.61 64.39 54.20 68.98 58.90 89.33
Gק×RS/No·m-2 860.61 734.26 968.50 420.14 339.00 1131.40 551.33 1196.75
%òpºDMC/% 24.76 26.91 24.94 26.29 27.32 24.69 23.89 25.36
s‘~„ WSC/% 3.16 3.80 3.35 4.38 2.90 3.10 3.65 2.91
qòCP/% 16.63 15.56 16.40 16.95 15.19 20.48 23.92 16.30
ëTP/% 0.23 0.18 0.22 0.22 0.28 0.25 0.29 0.24
=~ •uvNDF/% 62.71 62.85 64.21 60.99 63.22 61.89 57.34 62.87
/~ •uvADF/% 40.15 40.84 39.42 38.51 37.40 38.44 35.06 47.23
3 ™š
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,`
ém9
Ýþlòº
[20]。
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Y5Á

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¢ðs
Hž#}lXY&

zò5`…žð^5`
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Ié(¬+þ?–95ªlú«

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°2p^q?–^_`?Ž·¸Æb

Â^5
`lh¢þò

=>)“5®°P€î<5`óô
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[22],
YÌî<5
`”F^_?––˜€#7¾GÝ=lÏÊ

”g
±uöî<5`‚ˆÞ\lKLÍ

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lá§<5`

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‹
[23]。
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¿`?óŽlQ‹`J

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þò

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±SÿÙ^Ý\^þòzê~~lŠ§½)

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+~~¡|[ˆ‰lÈa

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I»¼mÆ;•?x£

ˆ‹÷Š7xx£e&1


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l‡ÂÍc|[Ä:‚]7"FÊôlñ[Š7x

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¾Jò½)lØMvw˜
¶Š§l±ËÂÃ<)xMlÖ¤
[25]。
&1x£lg
Y𣤽)ùçV.ol(

ö&1x£s˜
ö6£¤1·ùlçVaê`s˜+y¤ò1·ŸJ
ò½)ùlçVMÝ~
[26]。
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MÝ=I—_Ù#ê~êÙ5~

¡¢¢HJò½)
vw%±¿’

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7%w°@

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~~lʺ#~

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Vx78[1·,¢ð”&1ÞFÙ½)ØÙ-Ø
‡-JC?&”òQ

ÌðqXM¤Gl½)¦ƒ
&”+òQ

IA¸M¡
[27]
”DEäå`!Ž´G
p·È|}~ÞÆÇò*

ÈÉâãäå`FJò
½)ùlçVkêA&Ùf(=I

ŒÆÇqrŠ
7xx£lÆÇA&1x£MÝ

Š7xx£%±S
ll°„x6hI(H1l9ºJò½)=‘Ids
lP22,P23eP42&”+ò1,vId^lP16,P30,
P31,P36,P38eP39ñùçVaêd。£Æ°Æ%
wˆax£

%±SlI9º½)=P16s˜’¾^
݊§
,P22,P23eP42s˜’¾^„Š§,P36s˜
’¾^Š§ÖËÍò‰lZÔ±J¿’

ÌP30,
P31,P38eP39F~~Šodzês˜¢H»ò‰l
ÆB±±¥7_þJ

4+~~ùlM=~”±J¿’=ÿÙQ‹l
n¢wI

ž§òðZ%ùl¯’e!t

öäò
[~~q·NžM=~~lÈa
[28]。
âãäå`
çVÚÛYZ
[10],
£¤´GJò½)ñù4+~~
k]d(ýF[~~NoöUÖ¯

èe×Y~~
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˜œ×~~Ȟl°þòce((
Â^±J—²

§ST=

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M==Iúa

B^Aݺ

Gק×

%òpº
As‘~„pº

ëAqpº

/~ •uv
A=~ •uvñù¸7é!"vM==I

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”˜>l¿’=

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ëpº˜¶/~ •uvpº¡œ×ñ[4+~
~qSah(ºJò½)lî±%±

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Îsquvpº
ð°5`eßþò

Â^žh¢wIlQ‹Ÿ
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[29]。
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âãäå`Jò=%±Sòï~~Èazˆl½
)

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4 …š
  Ž¢Š7xx£e&1x£h45ºâãäå
`´GJò½)9[ݺeþò~~»¼l°vw
su

l°Èad|l½)Ù P30,P31,P38e
P39,iPs˜¢H»ò‰l±±˜¥7_þJ。
̌Æ[~~—rP16!H^ݽ),P22,P23e
P42!H^„½),P36!H^½),iPs˜
ÖËÍò‰lZÔ±J¿’

›[[1] FLW,ï&.=>5`±J¿’DE»«[J].=>4)

,2006,22(7):2326
[2] =>7,ëlÐ,+f.³/ãqŠ‹zò5`lJŽO›A
ݪú«ˆÊ
[J].BC<),2007,29(2):1520
[3] T:G,$ä,Rq:,¡.œ>5`JòBCq_DE»«
[J].ŽçVBC),2013,14(5):809815
[4] ïðJ,QGF,‚¼&.2p^q^_`?GHIJu¸T
el¯’z¬
[J].GH),2012,32(24):76887697
[5] ɱ,­ô:,ž­0,¡.2p^q¾9e9¶`9ãä~L
DE
[J].XYBC),2007,22(3):389397
[6] ïô,T³,äýD,¡.^_?–eºâãäå`½)h¢þ
òlod
[J].`ª<),2013,30(7):10891093
[7] 6>@,.2ã,ï¨Y.^_?–5ºäå`!5`4+~
~vw
[J].`?),2011,19(5):834838
[8] MiaoJM,ZhangXQ,ChenSY,犲狋犪犾.Gliadinanalysisof
犈犾狔犿狌狊狀狌狋犪狀狊Griseb.fromtheQinghaiTibetanPlateauand
Xinjiang,China[J].GrasslandScience,2011,57(3):127134
[9] RenF,ZhouHK,ZhaoXQ,犲狋犪犾.Influenceofsimulated
warmingusingOTConphysiologicalbiochemicalcharacteris
ticsof犈犾狔犿狌狊狀狌狋犪狀狊inalpinemeadowonQinghaiTibetan
plateau[J].ActaEcologicaSinica,2010,30(3):166171
[10]ChenSY,MaX,ZhangXQ,犲狋犪犾.Geneticvariationandge
ographicaldivergencein犈犾狔犿狌狊狀狌狋犪狀狊 Griseb.(Poaceae:
Triticeae)from WestChina[J].BiochemicalSystematicsand
Ecology,2009,37(6):716722
[11]ž>Õ,²„`,ô²,¡.ƒÏh^_`aGÝ9eŽ|}
~lém
[J].`ª),2012,21(2):305309
[12]DouQW,ChenZG,LiuYA,犲狋犪犾.Highfrequencyofkary
otypevariationrevealedbysequentialFISHandGISHinplat
eauperennialgrassforage犈犾狔犿狌狊狀狌狋犪狀狊[J].Breedingsci
ence,2009,59(5):651656
[13]·¯ã,au.âãäå`Ž¢wIvw[J].`ª<),2002,
19(9):1315
[14]ShiFS,WuY,WuN,犲狋犪犾.Differentgrowthandphysiolog
icalresponsestoexperimentalwarmingoftwodominantplant
species犈犾狔犿狌狊狀狌狋犪狀狊and犘狅狋犲狀狋犻犾犾犪犪狀狊犲狉犻狀犪inanalpine
meadowoftheeasternTibetanPlateau[J].Photosynthetica,
2010,48(3):437445
[15]6>@,.2ã,lWG,¡.^_?–´Gâãäå`4+~
~¶GÝ~evw
[J].=>`?),2011,33(6):5156
[16]FengRZ,LongRJ,ShangZH,犲狋犪犾.Establishmentof犈犾狔犿狌狊
狀犪狋犪狀狊improvessoilqualityofaheavilydegradedalpinemeadowin
QinghaiTibetan[J].Plantsoil,2010,327(12):403411
[17]ô³»,=M¨,[y,¡.(î4+¶Ýº~~lŠ7xx£
[J].(î<),2008,27(1):6773
[18]Ju¡,O–[,› £.56£¤p·F`Š‹4+~~
vw
[J].`?),2013,21(3):626630
[19]sŠ,Rd,gњ,¡.3J?§Ü¦^cdòe‡ûqr
òh%te¦UVB´µlmn[J].`?),2013,21
(3):517525
[20]RLX,ÁCÜ,­´_,¡.2p^q?–£¤âãäå`p
·eßþòDE
[J].`ª),2013,22(1):3845
[21]äýD,T³,ÁCÜ,¡.^_?–âãäå`zêJòBC

[J].=>4ª<),2013,46(4):841848
[22]¬µ,›²¶,·_G,¡.^_?–î<5`PJ°T[J].
=>`?
,2001,23(3):1822
[23]³KÜ.2385`±JDE»«[J].`ª<),2009,28
(11):8692
[24]ä"Q,+LS,U",¡.ÓGþJ(I)G)~~lŠ7
xx£e&1x£
[J].ÓG),2007,36(2):2834
[25]Þ¸,ѹJ,JXº,¡.£¤(ÎþJ(I)”«1?–lu
n~vw
[J].`?),2013,21(2):272279
[26]_ï=,¸ óœ,,g2,¡.WÒzêJòݺeþò~~Š
7x&1x£Al°vw
[J].=>4ª<),2008,41(9):
28592867
[27]¸M,Ȩ»,>*W.äå`!´Gp·È|}~¶žA
&=IDE
[J].6/Ž),2013,33(5):10271033
[28]‚óô,$\É,1ÈÈ¡.6•JòBCŠ‹Ž)~~l
çV|}~¶M=~x£
[J].ŽçVBC),2012,13
(1):1115,21
[29]FulkersoWJ,DonghyDJ.Plantsolublecarbohydratere
servesandsenescencekeycriteriafordevelopinganeffective
grazingmanagementsystemforryegrassbasedpastures:are
view[J].AustralianJournalofExperimentalAgriculture,
2001,41(2):261275

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