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Effects of Different Concentrations of Hormones on the Regeneration System of Tall Fescue

不同浓度激素配比对高羊茅再生体系建立的影响



全 文 :!23" !3#
 Vol.23  No.3
! " # $
ACTA AGRESTIA SINICA
   2015$  5%
  May.  2015
犱狅犻:10.11733/j.issn.10070435.2015.03.013
€u)œ^“ÁËvKžÑ/0‡ŸoÔÕ
øùú
1,
U+O
2,
ûüý
2,
 þ2
(1.1.à.ñí-../,1. b¦750021;2.1.à.®./,1. b¦750021)
67

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›Õ‰ÑúIÑYMòñùÔ«¶ž6!ñ2ù

€a+›

zcùԋŸ"«2{Ö_ MS+
5.5mg·L-12,4D+500mg·L-1CH;zcJ\"«2{Ö_ MS+2.0mg·L-16BA+1.0mg·L-1KT;zc
ñˆ"«2{Ö_1/2MS+0.3mg·L-1IBA,|ûü¯"_20g·L-1。
89:

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!ñ2ù
;<,-=
:S543.9    >?@AB:A     >CD=:10070435(2015)03051709
犈犳犳犲犮狋狊狅犳犇犻犳犳犲狉犲狀狋犆狅狀犮犲狀狋狉犪狋犻狅狀狊狅犳犎狅狉犿狅狀犲狊狅狀狋犺犲犚犲犵犲狀犲狉犪狋犻狅狀
犛狔狊狋犲犿狅犳犜犪犾犉犲狊犮狌犲
GUOLingna1,LIUJianwen2,MADongmei2,XUXing2
(1.NingxiaUniversity,Schooloflifescience,Yinchuan,Ningxia750021,China;
2.NingxiaUniversity,Schoolofagriculture,Yinchuan,Ningxia750021,China)
犃犫狊狋狉犪犮狋:Theimpactsofmanifoldfactorsoncalusinductionfrom matureseeds,subcultureproduction
andembryogeniccalidifferentiationwerestudiedhere.Plantregenerationsystemoftalfescue(犉犲狊狋狌犮犪
犪狉狌狀犱犻狀犪犮犲犪Schreb.)embryogeniccaliwasestablishedusingthesterileseedsofArid3varietyasex
plants.TheresultsshowedthatthebestcalusinductionmediumwasMS+5.5mg·L-12,4D+500
mg·L-1CH;andMS+2.0mg·L-16BA+1.0mg·L-1KTmediumperformedthebestfunctioninthe
differentiationoftalfescueembryogeniccali.Thebestrootingmediumwas1/2MS+0.3mg·L-1IBA,
andthecontentofsucrosewas20g·L-1.
犓犲狔狑狅狉犱狊:Turfgrass;Tissueculture;Regenerationsystem
EFGH
:20140520;IJGH:20140831
KLMN
:¸
¹
“973”¿¥(2012CB114204);_T@*];`ß¿(NYYZ2014Z12)ÀÁ
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(1989),á,1.8G§,úËÌÍñ,ÌÍփ_ž&2üRS,Email:guolingna2008@163.com;:6x× Author
forcorrespondence,Email:xuxingscience@126.com
  ÑYM(犉犲狊狋狌犮犪犪狉狌狀犱犻狀犪犮犲犪Schreb.)ûÍÞ
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)³3U’(犉犲狊狋狌犮犪犪狉狌狀犱犻狀犪犮犲犪‘Ar
id3’)` r݄Àë#$]÷â@›r¦R。
1.2 Ö×VW
1.2.1 ÿyº‹zFÿy!" ?íîn¤2?
"«2v_ MS"«2。1/2MS"«2à"Ê¥
_ MS"«2I¥x,|‘nJ‹—,‡ú¬30
g·L-1ûü,€8g·L-1,pHð_5.8。
"«NO

ye25℃,=Š#16h,=à/e
! " # $ !23"
3000~4000lx。
1.2.2 #J$@ÂÃ p“nI}2IÑYM‘)
³3U’]Ç]`r,ì%Š_’XI`r,õ”ïËÌI`ròÞ=$»8

>¤50%I‡¼
½H\]Ç]`r

Pf200r·min-1IY|†Â
ugc30min,ö¢¤_:ÉìO5~7~,!~ì
%&fɆŽI`r

!W]Ç]`ròÞ4℃
êR8;ÏË5

p“aË5¢]Ç]`r

>¤75%I~”Â
ugc30s,!¤0.1%÷(Âugc8min,z¢
¤ÿcÚÙÉìO5~7~,Wÿc]Ç]`rO`
Ø MS"«2,Þ25℃,3000~4000lx=à"«6
ׁ"«7d€。
1.2.3 %&H@()È*+ WÏ¡2I]Ç
]`rx_Cž2O`Ø*ú⋌–e2,4Dj
6BA{tIùÔ«¶‹Ÿ"«2†(+1),2,4D
14A–e,‘A–e‹ò3A~¡,2,4D j
6BA42A«,‘A«‹ò4A~¡,‘A~
¡O`50ACž2,f"«NO"«,"«1
Š¢)‚

P‹ŸMnùÔ«¶

‘15d‰Ú¥~,
‰Úâ~
。30d¢ú¼ùÔ«¶‹Ÿæ,ùÔ«
¶‹Ÿæ%=‹ŸqùÔ«¶ICž2G/O`C
ž2²G×100%。
1.2.4 %&Hz, ùÔ«¶‰Ú2~¢,<“
ßc–

ŠJI#q=ùÔ«¶

}¬Ø*ú⋌
D¥‡e{tI MSJ\"«2(+2)†,20A
‹ŒD¥–e«

‘A«‹ò4A~¡,‘A~
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¥~
,40d¢ú¼‚IJ\=GP¼jùÔ«¶I
J\æ

ùÔ«¶IJ\æ%=J\q‚IùÔ«
¶G

O`IùÔ«¶G×100%。
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~3cm€I·âñ–J\1‚IùÔ«¶,%¬
Ø*úâ0.1,0.3,0.5,1.0,1.5,2.0mg·L-1I
IBA6× NAAQ‹Œûü‡eI1/2MSñˆ"
«28

f=àNO5Wñˆ"«

"«28ûü
‡e‹ˆ_10,20,30mg·L-1,ԈI·e·Ø3
~5cm€%Z。20d¢††!ñž6Iñˆ=
GP¼j!ñž6Iñˆæ

!ñž6Iñˆæ=
ñˆI!ñž6

%¬I!ñž6²G×100%。
1.2.6 .,/0 fñˆ"«28"«15d€
I!ñž6Ôâà"‹ˆˆÍñ

Ôñˆ”â

>
€Û»Æ*…2~3d,*…#h‘È*úÚÙÉ6
×_:ÉÞ"«2†

*…¢Ô…”é­g¢5
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49Wÿc!ñ4…¤;r“q

¤’É
Ø+ìOìüˆU"«2

ÓÞ¦ág2cIY

Q
«Y=1:1IL©8,­• GÉé3,òÞ25℃,
=à16hIyÞ8ñ·。
q1  ¡ß¢£“ÉéK
Table1 Inductionmediumforcalus
"«2U
CulturemediumNO.
D¥Éu2,4D
Hormone
level2,4D/mg·L-1
D¥Éu6BA
Hormonelevel
6BA/mg·L-1









10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
3.0
3.0
3.0
3.5
3.5
3.5
4.0
4.0
4.0
4.5
4.5
4.5
5.0
5.0
5.0
5.5
5.5
5.5
6.0
6.0
6.0
6.5
6.5
6.5
7.0
7.0
7.0
7.5
7.5
7.5
8.0
8.0
8.0
8.5
8.5
8.5
9.0
9.0
9.0
9.5
9.5
9.5
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
815
!3# .ýž:‹Œ‡eD¥{t<ÑYM!ñ2ùÕ1IÕÖ
q2  ¡ß¢,)ÉéK
Table2 Differentiationmediumforcalus
"«2U
Culturemedium
NO.
D¥Éu2,4D
Hormone
level2,4D/mg·L-1
D¥Éu6BA
Hormonelevel
6BA/mg·L-1
D¥Éu
Hormone
levelNAA
/mg·L-1









10
11
12
13
14
15
16
17
18
19
20
0.0
0.0
0.0
0.0
1.0
1.0
1.0
1.0
2.0
2.0
2.0
2.0
3.0
3.0
3.0
3.0
2.0
2.0
2.0
2.0
0.0
1.0
2.0
3.0
0.0
1.0
2.0
3.0
0.0
1.0
2.0
3.0
0.0
1.0
2.0
3.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.5
1.0
1.5
2.0
2 mnU,c
2.1 €Éé_`Á ¡ß¢£“oÔÕ
WÑYM

)³3U’nI`rÏ¡Ë5¢,O
ØùÔ«¶‹Ÿ"«2†

f16h=àŠ#,(25±
1)℃NOI=à"«R86×fX"«NO
"«20d€,f=à¶XNO<ùÔ«¶‹
ŸÍñIÕÖ

ª+3。
q3 €Éé_`Á ¡ß¢£“oÔÕ
Table3 Effectsoflight/darkoncalusinduction
»`
Name
ùÔ«¶‹Ÿæ
Calus
inductionrate/%
òñùÔ«¶‹Ÿæ
Embryoniccalus
inductionrate/%
=àLight "«Dark =àLight "«Dark
)³3U
Arid3
88.45 90.00 29.34 38.29
Ý+3vPÚq,=ඁ"«NOùÔ«
¶‹Ÿæã*‹à

-˜XNOòñùÔ«¶
‹Ÿæt=àNOIòñùÔ«¶‹ŸæVÑ

¸ø.×#횰¤fXNO‹ŸMnùÔ«
¶IÖ³
[2],
<˜ü_]Ç]`rfXNO

Ô
»’MnvøtS+MIc\…

|Jñô.S/

#Î0‹ŸMnòñùÔ«¶

üNf“î;S8
zc"«NO_XNO‹ŸMnùÔ«¶

2.2 ¤¥¦iÁ ¡ß¢£“ÒÑoÔÕ
WÑYM

)³3U’` rO`ÞùÔ«¶‹Ÿ
"«2†

"«1Š¢`rÛÜ·q„‚,)ì`
r2UI„‚

P‹ŸMnùÔ«¶

fJ*)‚
1,2,3~g¢,ú¼ùÔ«¶‹Ÿæ,ß+4€an
ÅfùÔ«¶‹Ÿ;S8
,`
r·q„‚g¢)‚
3~g¢ùÔ«¶‹ŸæzÑ(‰1)。
q4 €¤¥¦iÁ ¡ß¢£“ÒÑoÔÕ
Table4 Theeffectsofdifferentchokebud
timesoncalusinduction
)‚~G
Chokebudtimes
ùÔ«¶‹Ÿæ
Calusinductionrate/%



50
52
54
<1 ¤¥3¦§o ¡ß¢
Fig.1 Calusafterthreechokebudtimes
2.3 €y)2,4犇Á ¡ß¢£“oÔÕ
fùÔ«¶‹ŸÉù
,2,4D_zâ>Iž&

[34]。
WÑYM

)³3U’` rO`Ø*úâ
‹Œ‡e–e2,4DIùÔ«¶‹Ÿ"«2†,"
«2J**ú‡e3.0,3.5,4.0,4.5,5.0,5.5,
6.0,6.5,7.0,7.5,8.0,8.5,9.0 j 9.5
mg·L-12,4D。*ú‹Œ–e2,4D¢,f‚2U
q,‹ŒFGIÝҖùÔ«¶

‰2)。
<2  ¡ß¢
Fig.2 Calus
915
! " # $ !23"
ÑYM

)³3U’` rf"«28‹Ÿ¥A%
¢IùÔ«¶ß,E›É,=

€›nÉ

™òñù
Ô«¶vø

-f‰Ú"«28‰Ú2~¢,øG
ßhc–

¹h

ŠJ

#q=IùÔ«¶

òñùÔ
«¶ùø

fùÔ«¶‹ŸÉù
,2,4D‡ef3.0~9.5
mg·L-1ghŽ,ùÔ«¶I‹Ÿæ"#2,4D‡
eI>÷Ñ¢ƒ

f2,4D_5.5mg·L-1Žù
Ô«¶‹ŸæzÑ_67%,ÑÞ2,4D‡e_3.0
mg·L-1ŽI48%(+5);ùÔ«¶Iñ·=òf
3.0~9.5mg·L-12,4D‡eLMåß,‹Œ=
ò

"#2,4D‡eI÷Ñ,ùÔ«¶¯É"†‡,
Ý34—Kœ…¹h

#qñù/

·•ùR

f2,
4D‡e_5.5mg·L-1ŽùÔ«¶øGß,hc
–

ŠJI#q=

òñùÔ«¶ùø

<ùÔ«¶
I‹ŸÕ֜‹
(P<0.05)(‰3)。üN,fùÔ«
¶‹ŸÉù

pffùÔ«¶‹Ÿ"«28*ú5.
5mg·L-12,4Dz©ªÞùÔ«¶I‹Ÿ。
q5 €y)2,4犇ᑨ ©3=’„ˆo ¡ß¢£“N
Table5 ThecalusinductionratesofArid3
atthedifferentgradientsof2,4D
"«2
Medium
D¥Éu2,4D
Hormonelevel
2,4D/mg·L-1
ùÔ«¶‹Ÿæ
Calus
inductionrate/%









10
11
12
13
14
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
48
50
52
62
65
67
60
58
57
48
49
50
53
55
<3 €y)2,4犇ᑨ ©3=’„ˆo ¡ß¢£“N
Fig.3 ThecalusinductionratesofArid3atthedifferentgradientsof2,4D
•

-å1~14J*Ú++58I1~14
Note:1~14arethesameasthe1~14oftable5
2.4 ,ª^6犅犃Á ¡ß¢oÔÕ
fùÔ«¶‹ŸÉù

ú¬+ÍJa¥<ùÔ
«¶‹Ÿïx¤

_‰o\«¶"«NO

fùÔ
«¶‹Ÿ"«28ü‰ú¬ñ·D¥2,4DC,‡
Cú¬0.0,0.1j0.2mg·L-1=A–eI+ÍJ
a¥6BA,P‹<àxtS,††ùÔ«¶Iñ·
FG

ß+6nÅ:¶<૬t,ú¬6BAI"«
28

ÑYMIùÔ«¶‹Ÿæù’ñ‹ŒSeI
ƒ

æçf2,4D‡e¬ŒŽ,"#6BA‡eI
ք

ùÔ«¶I‹Ÿæœ…ƒ

Pçfíî;S
8’,ú¬6BA P¢IùÔ«¶ßÉ,=,€›


™òñùÔ«¶ùø

‹£Þ¢°ùÔ«¶J
\n…
。6BA‹˜ùÔ«¶‹Ÿ;S8ImVü
¥

<ùÔ«¶‹Ÿx¤S4

<¶;µ›IÌÍ
€a¥Š
[5]。
üN

fùÔ«¶‹ŸÉù

‹ú¬+
ÍJa¥6BA >a¬2。Pçe2,4D‡e_
5.5mg·L-1Ž,ùԋŸæzà(犘<0.05)。
025
!3# .ýž:‹Œ‡eD¥{t<ÑYM!ñ2ùÕ1IÕÖ
q6 6犅犃Á ¡ß¢£“oÔÕ
Table6 Theeffectofcytokinin6BAoncalusinduction
"«2U
Culturemedium
NO.
D¥Éu2,4D
Hormone
level2,4D
/mg·L-1
D¥Éu6BA
Hormone
level6BA
/mg·L-1
ùÔ«¶‹Ÿæ
Calusinduction
rate/%









10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
3.0
3.0
3.0
3.5
3.5
3.5
4.0
4.0
4.0
4.5
4.5
4.5
5.0
5.0
5.0
5.5
5.5
5.5
6.0
6.0
6.0
6.5
6.5
6.5
7.0
7.0
7.0
7.5
7.5
7.5
8.0
8.0
8.0
8.5
8.5
8.5
9.0
9.0
9.0
9.5
9.5
9.5
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
0.0
0.1
0.2
48.00±1.73HIhi
25.75±0.24TUv
23.55±0.55Vw
50.00±1.00Hh
25.00±0.46Uv
24.50±0.46UVvw
52.00±0.42Gg
43.25±0.03Kk
31.45±0.49Pr
62.00±1.00Cc
40.35±0.13Lm
23.45±0.72Vw
65.00±2.00Bb
34.55±0.07Np
28.00±0.40RSs
67.00±0.09Aa
62.50±0.50Cc
45.00±0.17Jj
60.00±0.01Dd
41.65±0.88Ll
38.00±0.50Mn
58.00±0.35Ee
40.00±1.06Lm
33.00±0.87NOq
57.00±0.10Ee
38.85±0.18Ln
29.50±0.62Qs
48.00±1.11HIhi
32.65±0.31OPq
27.00±0.17Sst
49.00±0.56Hh
29.00±0.66QRs
25.45±0.13TUv
50.00±0.15Hh
26.85±0.09STu
20.15±0.13Wx
53.00±0.08Gg
34.35±0.08QRp
26.75±0.15STuv
55.00±0.26Ff
36.65±0.16Mo
27.00±0.17Sst
2.5 €œ^u)klÁ ¡ß¢,)oÔÕ
fùÔ«¶J\;S8

+ÍJa¥ï#·­


_‰‡ˆùÔ«¶J\"«2Iz©*ú+
ÍJa¥`aj‡e

WòñùÔ«¶O`Þ*ú
⋌–e6BA j KT«IJ\"«2†,f
=à16h·d-1INO,"«30d¢,߉4nÅ
ùÔJ\qñÝ6ñ‚

*ú‹ŒD¥{tIùÔ
«¶‹ŒSeIJ\qñÝ6×ñ‚

<4  ¡ß¢o,)
Fig.4 Calusdifferentiationofembryoniccalus
  MS"«28*ú‹Œ‡e{tI6BA,KT
jNAA<ùÔ«¶J\n…✋ÕÖ。‹ŒD
¥‡e{t<ùÔ«¶J\IÕÖß+7nÅ:¶
<૬t

eKT66BAÇ0£¤Ž,f0~3.0
mg·L-1gh,ùÔ«¶IJ\æ⋌SeI¦
Ñ

eKT66BAI‡eùàÞ3.0mg·L-1Ž,
ùÔ«¶IJ\æÛ܍Pçq,‹ŒSeI.
\,Ä

e‹ŒI2`+ÍJa¥{Ž,’,
6BA¶KT{£¤Ž,<ùÔ«¶J\âҌù
>x¤
,2`D¥{t©ô‡5J\,æ6BA¶
NAA{£¤Ž,J\æ¶ KT{£¤Sƒ,ç
ùÔ«¶ß,É,=

‹Î0J\qñ…



f
ùÔ«¶J\Éù 2.0 mg·L-1 6BA+1.0
mg·L-1KT<ùÔ«¶J\Õ֜‹(犘<0.05)

‰5),30d¢,ùÔ«¶J\qñ‚,J\æzÑ
v}Ø69.87%。
2.6 €œ^1«¬u)klÁžÑˆjÑëN
oÔÕ
ÑYMf‹*úˆËD¥I"«2†ÛvPñ
ˆ

-˜_‰¦ÑñˆæPQŸHñˆŽh

?íî
WJ\qI!ñž64…J*}¬Ø¯â‹Œ‡e
IBAI1/2MS"«2†,ñˆ"«28ú¬IBA,
Ô
‰6A、B)。
‡Cûü˜«¶"«8š¤IÄ?

#˜¦Rô"
I:?

iïØ[E•Tˆ)Ix¤
[6]。
fÑYM
ùÔ«¶‹Ÿ;S8

Ï+88vPÚq,IBA‡e
_0.1mg·L-1,ûü‡e_10g·L-1ŽñˆŽ
125
! " # $ !23"
hS ·

ñ ˆ æ # S ƒ

f IBA ‡ e _ 0.3
mg·L-1,ûü‡e_20g·L-1Ž,"«2Š€
ñˆævP}Ø100%,ñˆnUŽh#SH,Î0
Íñà"ш

fIBA‡e_2.0mg·L-1,ûü
‡e_30g·L-1Ž#vPñˆ,-˜ñˆæ›œ
_76%,ñˆŽhÔân$/。‡CÝ+9v
PÚq

ú¬IBA6 NAA¬t‹úD¥vP¦Ñ
ñˆæ

|8IBAtNAA¬2。üNfñˆŽp
×f"«28*ú0.3mg·L-1IBA,20g·L-1û
üvP¦Ñ!ñž6Iñˆæ

ŸHñˆŽh

<5 €u)6犅犃、犓犜Á ¡ß¢,)NoÔÕ
Fig.5 Theeffectsofdifferentconcentrationsof6BAandKToncalusdifferentiationrate
•

-å1~20J*Ú+781~20
Note:1~20arethesameasthe1~20oftable7
q7 €u)6犅犃、犓犜Á ¡ß¢,)NoÔÕ
Table7 Theeffectsofdifferentconcentrationsof
6BAandKToncalusdifferentiationrate
"«2
Medium
6BA
/mg·L-1
D¥Éu
Hormone
levelKT
/mg·L-1
D¥Éu
Hormone
levelNAA
/mg·L-1
J\æ
Calus
differentiation
rate/%









10
11
12
13
14
15
16
17
18
19
20




1.0
1.0
1.0
1.0
2.0
2.0
2.0
2.0
3.0
3.0
3.0
3.0
2.0
2.0
2.0
2.0

1.0
2.0
3.0

1.0
2.0
3.0
0.0
1.0
2.0
3.0
0.0
1.0
2.0
3.0




















0.5
1.0
1.5
2.0
22.85
31.56
59.68
49.46
25.65
30.73
48.32
31.47
43.92
69.87
40.36
23.45
30.68
34.36
28.64
10.65
42.35
62.54
60.34
41.65
<6 ­K‡žÑˆjoÑë
Fig.6 Thefibrousrootsofthereplantedplants
•
:A:}2ü!ñž6;B:!ñž6Iˆù
Note:A:Replantedtransgenicplants;B:Rootsofthereplantedplants
2.7 žÑˆjo®¯
fñˆ"«28"«15d€I!ñž6Ô
âà"‹ˆˆÍñ

Ôñˆ”â

>€Û»Æ*…
2~3d,*…#h‘È*úÚÙÉ6×_:ÉÞ"
«2†

*…¢Ô…”é­g¢5W%Z

49
225
!3# .ýž:‹Œ‡eD¥{t<ÑYM!ñ2ùÕ1IÕÖ
Wÿc!ñ4…¤;r“q

¤’ÉØ+ìOìü
ˆU"«2

ÓÞ¦ág2cIY

Q«Y=1:1I
L©8

­• GÉé3

òÞ25℃,=à16hI
yÞ8ñ·

‰7)。
q8 €u)犐犅犃犖犃犃1«¬u)ÁžÑˆjÑëNoÔÕ
Table8 TheeffectsofdifferentIBA,NAAandsucroseconcentrationsontherootingrateofregenerationplant
"«2ab
Mediumtype
IBA/mg·L-1
Indole3Butytricacid
ûü
Sucrose/g·L-1
ñˆŽh
Timeofrooting/d
ñˆæ
Rootrate/%
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
1/2MS
0.1
0.3
0.5
1.0
1.5
2.0
0.1
0.3
0.5
1.0
1.5
2.0
0.1
0.3
0.5
1.0
1.5
2.0
10
10
10
10
10
10
20
20
20
20
20
20
30
30
30
30
30
30
24
22
21
23
22
25
20
16
17
18
20
22
23
22
23
25
23
25
86
100
98
96
90
80
90
100
97
95
92
91
88
96
94
87
80
76
<7 ­K‡žÑˆj®¯
Fig.7 Transplantsofthereplantedplants
•
:A:!ñž6;B:!ñž6I©Z
Note:A:Replantedplants;B:Rootingofthereplantedplants
q9 €u)犐犅犃犖犃犃ÁžÑˆjÑëNoÔÕ
Table9 TheeffectsofdifferentIBA,NAAconcentrationsontherootingrateofregenerationplant
"«2ab
Mediumtype
IBA/0.3mg·L-1 NAA/1mg·L-1
ñˆæ
Rootingrate/%
1/2MS - - 80
1/2MS - - 65
1/2MS - - 55
1/2MS + - 90
1/2MS + - 75
1/2MS + - 60
1/2MS - + 75
1/2MS - + 55
1/2MS - + 50
  •:“+”+Åú¬N&À,“-”+Å/âú¬
Note:“+”meansadding,“-”meansno
325
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àøGž&I!ñ2ùý2üb

Cž2`a
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¥AÑ>!ñ2ù穤ÞHˆIx`2
übQCž2Iab
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`rx_Cž2‹ŸòñùÔ«¶
[10]。
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f‹Ÿ"«28*úD×¥
(KT )[11]、
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(ABA)[13]、NAA[13]j2

(TDZ)[13]<ùԋŸÕ֋œ‹。ˊUj
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PÑYMI4¥UJ_Cž2

ùÔ«¶‹Ÿ"«
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BaijQu[9]°¤ÑYM‘Coronado’I”ï`
rx_Cž2

fùԋŸÉù*ú‹Œ‡e2,4
D(2,5j9mg·L-1),’,|SoIùÔ«¶‹
Ÿ"«2_ MS+2,4D9mg·L-1,ùԋŸæ_
19%。BaijQu[12]f<ÑYM‘Coronado’»`I
!ñ2ùÕ18Ò~£¤ŽKIÖ³Ë5nIIÑ
YM`r

f2,4D‡e_5mg·L-1Ž,ŽK¢I
`r
(57.3% )˜”ï`r(14.7%)‹ŸæI4
Y

Ñ´Ë
[15]
¤ÑYM”ï`r‹ŸùÔIÌ
̀a+›

f2,5,7,9j12mg·L-12,4D8,
©ªùԋŸI"«2#_ MS+9mg·L-1
2,4D,-ùԋŸæÑ}68.08%。?“î+›Ñ
YMInI`r<2,4DI‡e.­‚ev³,f
1~9mg·L-1‡eLMåùô‹ŸqùÔ«¶,2,
4D‡ef3.0~9.5mg·L-1ghŽ,ùÔ«¶I
‹Ÿæ"#2,4D‡eI>÷Ñ¢ƒ,f2,4D
_5.5mg·L-1ŽùÔ«¶‹ŸæzÑ_67%,‹
ŸqIùÔ«¶øGß,hc–

ŠJI#q=

=
òzc

<¶JunshengZhao[16]IÌ̀a¥
Š

?ÌÍ©ªI2,4D‡e(5.5mg·L-1)¶á
§¸|‹¥Š

æùÔ«¶‹Ÿæ¶é¸|I€a
¬e

¾n<¥€avô˜ÝދŒIÑYM»`
ùÔ«¶‹ŸnUVI2,4D‡e‹Œ,‡¥ÖŽ
#˜ Ý Þ “ î ‹ ¼ I É u h « S 4
(0.5
mg·L-1),2,4D‡eLMS³(1~9.5mg·L-1)

üNôÛºpج©ª

¬%‡I2,4D‡e。
3.3 €œ^u)klÁ ¡ß¢,)oÔÕ
MS"«28*ú‹Œ‡e{tI6BA,KT
jNAA<ùÔ«¶J\n…✋ÕÖ。Baij
Qu[12]f<ÑYM‘Coronado’‹ŸùÔI!ñ2ù
8
,MS+2.5mg·L-16BAIJ\æ}Ø33.1%。
?Ì̀a+›

¶<૬t

eKT66BAÇ
0£¤Ž

f0~3.0mg·L-1gh,ùÔ«¶IJ
\æ⋌SeI¦Ñ

e KT66BAI‡eù
àÞ3.0mg·L-1Ž,ùÔ«¶IJ\æÛ܍
Pçq,‹ŒSeI.\,Ä

íî;S8õöù
ú+ÍJa¥¯"ô¦ÑÑYMùÔ«¶IJæ

-+ÍJa¥I¯"#‹ª;Ñ

,°ÔŸŠ!
ñ„…ñ·;R

ˆñ·ýØXO

!ñž6n!æ
ƒ

e‹ŒIâ`+ÍJa¥{Ž

’,6BA
¶KT{£¤Ž,<ùÔ«¶J\âҌù>x
¤

â`D¥{t©ô‡5J,
æ6BA ¶
NAA{£¤Ž,J\æ¶ KT{£¤Sƒ,ç
ùÔ«¶ß,É,=

‹Î0J\qñ…

3.4 €œ^1«¬u)klÁžÑˆjÑëN
oÔÕ
ûü˜«¶"«8š¤IÄ?

#˜¦Rô"
I:?

iïØ[E•Tˆ)Ix¤

чeûü
ôf¥ˆSe†XO™òñùÔ«¶Iñ·‡5

òñùÔ«¶Mn
[17]。
?íî+›fIBA‡e_
0.3mg·L-1,ûü‡e_20g·L-1Ž,"«2Š
€ñˆævP}Ø100%,ñˆnUŽh#SH,
Î0Íñà"ш

|ûüvô˜Ñ‡eûüj
€<ùÔ«¶Íñ‰¥`ÉJ6Q«4#

6×d
ïù Ô « ¶ E x ñ I ù ú

Ï æ ‡ 5 | ž 6

[1819]。
425
!3# .ýž:‹Œ‡eD¥{t<ÑYM!ñ2ùÕ1IÕÖ
4 m
?Ó:;<

)³3U’!ñ2ùÕ1IÌÍ,
|€a+›

P MS"«2_23,zcùԋŸ‡
e_5.5mg·L-12,4D+500mg·L-1CH;zc
J\‡e_2.0mg·L-16BA+1.0mg·L-1KT;
zcñˆ‡e"«2{Ö_1/2MS+0.3mg·L-1
IBA,ûü¯"_20g·L-1。
žŸ>?
[1] BarnesR F.Importanceandproblemsoftalfescue.In:
KasperbauerMJ(ed).Biotechnologyintalfescueimprovement
[M].CRCPress,1990:212
[2] VanderValkP,ZealMA.CreemersMolenaar.Somaticem
bryogenesisandplantregenerationininflorescenceandseed
derivedcalusculturesof犘狅犪狆狉犪狋犲狀狊犻狊L.(Kentuckyblue
grass)[J].PlantCelReport,1989,7(8):644647
[3] VanArkHF,ZaaM A,CreemersMolenaarJ.犲狋犪犾.Im
provementofthetissuecultureresponseofseedderivedcalus
culturesof犘狅犪狆狉犪狋犲狀狊犻狊L.:Effectofgelingagentandab
scisicacid[J].PlantCel,tissueandOrganCulture,1991,27
(3):275280
[4] ›˜X,–«ó,¬ä ,.HIŸI«¶"«j2ü5pŸ
I2ü}\2ùIfzÕ1
[J].¡6.¸(_ö-.Á),
1999,10(5):540544
[5] ;µ›,²³´,§·”.7C2]4HIŸùÔ«¶I‹Ÿ
Q!ñ2ùIÕ1
[J].8¸]4,2005,27(3):3136
[6] ™Óæ,8š.†9ž&«¶"«„…[M].„À:8¸W÷
qÁÂ
,1991
[7] WangZY,AndrewH,RoufM.Forageandturfgrassbiote
chonology[J].CriticalReviewinPlantScience,2001,20(6):
573619
[8] PrzetakiewiceA,OrczykW,NadolaskaOrczykA.Theeffect
ofauxinonplantregenerationofwheat,barleyandtriticale
[J].PlantCel,TissueandOrganCulture,2003,73(3):245
256
[9] BaiY,QuR.Anevaluationoncalusinductionandplantre
generationof25turftypetalfescue (犉犲狊狋狌犮犪犪狉狌狀犱犻狀犪犮犲犪
Schreb.)cultivars[J].GrassandForageScience,2000,55
(4):326330
[10]_›Â.]Ç]}2üÌÍ5“[J].-„:¸,2002,18(1):
7374
[11]Ëï,<¯—,˜:.ÑYMnIò‚2"«QÑúž6!
ñ
[J].]÷-.,2005,22(6):2329
[12]BaiY,QuR.Factorsinfluencingtissuecultureresponsesof
matureseedsandimmatureembryosinturftypetalfescue
[J].PlantBreeding,2001,120(3):239242
[13]AshokC,QuR.Somaticembryogenesisandplantregenera
tionofturftypebermudagrass:Effectof6benzyladeninein
calusinductionmedium [J].PlantCel,TissueandOrgan
Culture,2000,60(2):113120
[14]ˊU,Þ£.‹Œ`òQD¥Ë5<ÑYMùÔ«¶‹Ÿ
IÕÖ
[J].!Ÿ®÷-.,2003,31(3):371374
[15]Ñ´Ë,¬r;,–ÕÐ,.ÑYM«¶"«!ñ2ùQ
GUS2ü45
[16]ZhaoJ,ZhiD,XueZ,犲狋犪犾.Enhancedsalttoleranceoftrans
genicProgenyofta11fescue(犉犲狊狋狌犮犪犪狉狌狀犱犻狀犪犮犲犪)expressing
avacuolarNa+/H+ antiportergenefrom Arabidopsis[J].
JournalofPlantPhysiology,2007,164(10):13771383
[17]U(X,‰i›,A·”,.Ã=ànIòI«¶"«Qž
6!ñ
[J].]÷.¸,2003,12(1):8589
[18]ÞÒ,ì>,?¯›,.¦Ñ4EùÔ«¶J\úæIü¥
[J].ž&ñ5.¸,1999,25(4):388394
[19]~@ñ,»[A.1ÉË5<É¿«¶"«ž6!ñIÑ>D
]x¤
[J].®÷-.ʱ!ÃÊ(®x&ûñÀ2"«ß
u
),1995:3946

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525