摘 要 ：通过用一对特异性引物,PCR扩增得到花生(Arachis hypogaea)主要致敏蛋白Ara h 1的基因的启动子片段1957 bp,其序列组成与已发表序列基本一致。用其替换pBIN 35S-mGFP4载体中的35S启动子部分,组成一种花生转基因表达载体pGA1。该载体含有种子特异表达调控元件、增强表达元件和一些与转录因子结合的调控元件,是一个强启动子,便于将外源基因转入花生,实现外源基因在花生中的表达,为建立花生生物反应器奠定了基础。
Abstract:The promoter of the gene encoding the major peanut allergy protein Ara h 1 has been cloned by the polymerase chain reaction technique(PCR) with a pair of special primers.The promoter we have got is 1957 bp.Basically,it is consistent with the sequence published.By replacing the 35S promoter in pBIN 35S-mGFP4 vector with this promoter,we have constructed a plant transgenic expression vector of peanut named pGA1.It contains seed specifically expressed elements,enhancers and some elements regulated by transcription factors.Thus the heterologous gene can be transferred to peanut and expresses in it easily.The research lays a foundation for establishing the peanut bioreactor.