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作 者 ：张述祖;施良

期 刊 ：武汉植物学研究 1986年 4卷 4期 页码：363-366

关键词：雄性不育；组织培养；组织化学；菜豆；

Keywords:Male sterility, Tissue culture, Histochemistry, Phaseolus vulgaris,

摘 要 ：本实验利用铁氰化钾处理组织培养的菜豆早期花药(小孢子单核期),以检验该药剂的杀雄效果。在组织培养条件下,药剂杀雄的有效浓度为0.1—0.5ppm;通过组织化学及扫描电镜等细胞学观察表明,杀雄作用与绒毡层过氧化物酶受抑制及小孢子外粉壁形成受阻有关。

Abstract:The male sterile effect of potassium ferricyanide treatment was examined on the anther of Phaseolus vulgaris L. during the mononuclear microspore phase under tissue cultural condition.The range of the effective concentrations of the reagent is from 0.1ppm to 0.5ppm. By the observations of histochemistry and SEM, we can make a conclusion that the effect of sterilization is related to the inhibition of the activity of peroxidase in tapetum and the formation of the exine.

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