全 文 ： ACTA AGRONOMICA SINICA 2008, 34(2): 341−343 http://www.chinacrops.org/zwxb/
ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@chinajournal.net.cn
:  30600417
 : (1971–) !#$%&()*+,%-Tel/Fax: 023-68250383E-mail:
xsq@swu.edu.cn.
* /012(Corresponding author): 34-Tel/Fax: 023-68250383E-mail: lianggl@swu.edu.cn
Received(5678): 2007-06-19; Accepted(9:78;: 2007-08-27.
DOI: 10.3724/SP.J.1006.2008.00341
 Ipomoea trifida(4x) GISH
        *
( ,  400716)
 :  I. trifida (2x) , I. trifida (4x) 2“695104”“697288”
 !, #$%&, 2 I. trifida (2x)()*+, , 2-./0123“695104”4
56(78(9:;<-., = I. trifida (2x)>?@ABCDB “697288” “695104”EF, GH
I8J9(!-., ,-.KL ;<-., U 3 ZQKL(-., h ZQi23
: D D !(GISH)
GISH Analysis of Sweet Potato Wild Relative Ipomoea trifida (4x)
XIANG Su-Qiong, WANG Wei-Xing, LI Xiao-Lin, CHEN Yao, GUO Qi-Gao, HE Qiao, and LIANG
Guo Lu*
(Horticulture and Landscape College, Southwest University, Beibei 400716, Chongqing, China)
Abstract: Using genomic DNA of I. trifida (2x) as probe, I. trifida (4x) lines 695104 and 697288 were analyzed with genome in
situ hybridization (GISH). The results showed that the two liness were very closely related to I. trifida (2x) based on hybridization
signals. Bright and even hybridization signals were observed on all chromosomes of 695104, showing that I. trifida (4x) 695104
should be from doubling of I. trifida (2x) chromosome set. Hybridization signals of 697288 differed from those of 695104 in
brightness and region size. It displayed there types: the first was that 41 chromosomes appeared bright and even signals on whole
chromosomal; the second was that 14 chromosomes presented even signals almost on whole chromosome but weaker than the first;
the third was that 5 chromosomes displayed more weaker signals than those of both the first and the second. Therefore 697288
might derived from I. trifida (2x), but through recombination and variation.
Keywords: Sweet potatoDIpomoea trifida; Genome in situ hybridization (GISH)
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Table 1 Materials for this study

No. of accession

Female parent

Male parent
 
Chromosome number

Species
CIP693231 2x10.1 2x3.1 2n=2x=30 I. trifida (2x)
CIP697288 4x6.1 4x20.1 2n=4x=60 I. trifida (4x)
CIP695104 4x19.1 OP 2n=4x=60 I. trifida (4x)
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>45 30 min, ?×35% Giemsa( 5~10
min, @ABCDd•3
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2K, LK 5 minD1%.P(50 mmol L−1 MgCl2, 1×PBS,
)ƒQ‡45 5 min, 2×SSCRA 3K, LK 5 min3
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 1 I. trifida(4x)695104 697288 GISH
Fig . 1 GISH analysis of I. trifida(4x) 695104 and 697288
A: 695104 GISH; B: 695104 GISH; C: 697288 GISH;
D: 697288 GISH 5 µm
A: GISH result of 695104; B: GISH signal of 695104; C: GISH result of
697288; D: GISH signal of 697288 scale bars=5 µm.
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