利用改良的穗茎注射法将反义PLDγ基因导入普通小麦兰考906，经特异PCR扩增和PCR-Southern杂交技术筛选出转化体并分析研究了转化株系与受体性状的差异。结果表明: (1) 3个材料扩增出与阳性质粒大小相同的400 bp的目的片段，说明该基因已整合到小麦的基因组中，获得了转基因小麦植株。（2）转基因株系在农艺性状上与受体存在显著差异，主要表现为株高、千粒重、单株穗数增加。变化范围分别为79.2～83.8 cm、50.53～52.98 g、5.30～8.86穗，分别比对照提高13.4 cm、6.94 g、3.82穗；而穗长、小穗数、穗粒数减少。（3）转基因株系的成熟期明显比受体提早4~8 d；转化株系早代和后代中均存在完全的雄性不育现象。
Antisense phospholipase Dγ (PLDγ) gene was introduced into LK906 wheat mediated by improved earstem injecting of Agrobacterium. The transformants were screened by PCR and PCR-Southern technique and the variation between transformation lines and receptor was analyzed. The results indicated that（1）400 bp special fragment to the plasmid was amplified in three plants（03039,03048,03050）, which showed that anti-PLDγ gene was integrated into wheat genomes, thus the transgenic wheats of anti-PLDγ gene were obtained in the world first. (2) There were distinct differences between transgenic lines and receptor in agronomic characters, such as plant height, 1 000-kernel weight and spike number per plant that were higher in transgenic lines than in receptor. Compared with in the receptor, in transgenic lines the plant height was from 79.2 cm to 83.8 cm and 13.4 cm higher; the average value of spike number per plant was 3.82 and higher significantly too; and 1 000-kernel weight was from 50.53 g to 52.98 g and 6.94 g higher. While the spike length, number of spikelet and grain number per spike were lower (Table 1). (3) The mature date of CK was on June tenth, those of transgenic plants (03048,03039,03050) were on June second, June third, June sixth respectively, and 4 to 8 days shorter than that of receptor. It showed that anti-PLDγ gene was good to advance mature date. Complete male sterile was observed in T1 and the following generations and it would play an important role in heterosis utilization.
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