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作 者 ：何启谦;刘新芝

期 刊 ：作物学报 1994年 20卷 01期 页码：33-38

关键词：苜蓿；组织培养；叶绿体DNA；线粒体DNA；

Keywords:Alfalfa, Tissue, culture, Chloroplast DNA Mitochondrial DNA,

摘 要 ：用差速离心法自继代培养5个月的愈伤组织中分离叶绿体和线粒体。用饱和酚、氯仿和氯化铯梯度离心的方法纯化二种细胞器的DNA。同时，用同样的方法自供体植物叶中分离和纯化叶绿体和线粒体DNA。采用了6种限制性内切酶、凝胶电泳和Southern印迹杂交的方法，比较了供体植物和愈伤组织中叶绿体DNA和线粒体DNA酶切谱带的变异情况。

Abstract:Chloroplast DNA and mitochondrial DNA were isolated form leaves and callus of alfalfa, Those DNAs were digested with six restriction endonucleases (BamH1,Bg1 1, Bg1 II, Dra 1, EcoR1, HindIII), electrophoresed and transferred to nylon membianes. Southern blots were hybridized with sorghum probes cont

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