Abstract:This study of tissue culture of Pyrethrum cinerariifolium Trev. has been on basis of selected 40 clones which progressed for several years with the current teamer. We sought to make new ways that could be more fast breeding for commercialize development and preservel these materiala of clones. Through a series of experiment, it has found that to take bud -shoots vaccinated and induced sprouting in MS medium diredy could obtained a fine result. As a general rule from pick down the bud- shoots to germination in MS medium, these has always needs 30-40 days, on these basis it has been into next continual growing stage until taken the young plants transfer to fields, this course as usually needs 110 ~ 135 days, it would be benifit increase in survival rate.