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期 刊 ：植物遗传资源学报 2014年 15卷 6期

关键词：小麦；醇溶蛋白；多样性分析；品质；相关性；

Keywords:wheat, gliadin, genetic diversity, quality, correlation,

摘 要 ：摘要：为了解67份美国材料的遗传多样性及其醇溶蛋白亚基对品质性状的影响，利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)技术对这67份材料进行醇溶蛋白谱带分析，测定了供试材料的面团流变学特性及理化品质。结果表明，在67份美国材料中共分离出1332条谱带，49种不同迁移率类型的谱带，大部分谱带具有多态性。单个材料谱带总数变异幅度为13～28。谱带数在α、β、γ、ω 4个区的分布存在较大差异。没有发现电泳谱带完全相同的材料。67份美国材料GS值变异范围0.54～0.90，平均值为0.731。在GS值为0.607水平上，聚类分析将这67份材料分为6类。49条不同迁移率的谱带中有17条谱带与36项品质性状的相关性达到显著差异或极显著差异。6条谱带(迁移率为49.6、56.2、56.7、62.2、79.4、86.8)与湿面筋含量、蛋白质含量、和沉淀值呈正相关，而迁移率为60.5的谱带与之呈负相关。11条谱带（迁移率为26.5、42.0、49.6、52.5、56.2、56.7、62.2、64.1、72.0、79.4、86.8）与面团稳定时间、面团形成时间、延伸面积等面团流变学特征呈正相关，而迁移率为34.4、47.5、49.0、60.5、69.4、85.4的这6条谱带则与之呈负相关。说明了供试材料间存在着丰富的遗传多样性以及与优质品质相关的谱带，对进一步利用这67份种质资源和为优质小麦品种的选育提了提供理论依据。

Abstract:Abstract: In order to know the diversity in 67 American wheat germplasm resources and investigate the effects of gliadin variation on wheat quality characters, A-PAGE was used to analyze the variations of prolamin among those wheat germplasm resources. moreover, their farinograph and physical chemistry qualities were tested. The results showed that there were 1332 protein bands in tested germplasms and there were 49 protein bands with different mobility. Most of those different mobility bands showed great polymorphism. Each material had 13 to 28 bands. The number of bands in α、β、γ and ω zones had big difference. Different material has different bands. The genetic similarity ranged from 0.54 to 0.90,with an average of 0.731.Cluster analysis based on genetic similarity of seed gliadins showed that 67 cultivars could be divided into 6 clusters at the level of GS 0.607.Significant or extremely significant correlation were found between 17 of the 49 bands and 36 kinds of wheat quality characters.6 bands(The mobility were 49.0、49.6、56.2、56.7、79.4、85.4、86.8) were positively correlated with protein content, gluten content and zeleny , while 60.5 was negatively correlated with protein content, gluten content and zeleny. 11 bands(The mobility were 26.5、42.0、49.6、52.5、56.2、56.7、62.2、64.1、72.0、79.4、86.8) were positively correlated with good wheat farinograph of dough stable time, development time and Stretch area, etc., while another 6 bands (34.4、47.5、49.0、60.5、69.4、85.4）showed opposite trend. High genetic diversity in 67 American wheat germplasm resources was observed and bands which were positively correlated with good properties were existed, which would provide theoretical guidance for the further use of those 67 germplasm resources and high-quality wheat varieties breeding.

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