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作 者 ：庄承纪 梁汉兴

期 刊 ：云南植物研究 1985年 7卷 04期 页码：1-3

关键词：金花茶；组织培养；胚状体；

Keywords:Camellia chrysantha,

摘 要 ：研究了金花茶（Camellia chrysantha（Hu）Tuyama）子叶在离体培养中体细胞胚状体发生的条件。在MS基本培养基中附加苄基嘌吟（BA）或苄基嘌吟与萘乙酸（NAA）组合，诱导了胚状体发生。组织学观察表明，胚状体起源于子叶的表皮细胞。在增添细胞分裂素和生长素的MS或改良B5液体培养基里振荡培养，明显地促进了胚状体根的生长和茎的发育。胚状体在继代培养中能保持旺盛的再生能力。已得到两个繁殖率较高的胚状体无性系。在合适的条件下，胚状体能长成正常的小植株。

Abstract:The conditions of somatic embryogenesis in cothledon culture of Camellia chrysantha(Hu) Tuyama were studied.The embryoids were occurred on the Murashige and Skoog medium with the addition of 6-benzyladenine or combination with NAA.Histological observations showed that the embryoids originated from the epidermal cells of cotyledon.The root growth and the shoor development of embryoids were promoted markedly in the MS or B5 liquid medium wupplemented with cytokinin and auxin by shake culture.

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