Abstract:Composition and contents of eight isoquinolide alkaloids stepholidine(l), rutodine(2), crebaninc( 3 ), isocorydine( 4 ), dicentrine( 5 ), cepharanthine( 6 ), tetrandrine(7) and cycleaninein ( 8 ) in nine species of Stephania plants from yun-nan and Guizhou which were analyzed by high performance liquid chromatogr-aphy (HPLC) on a Zorbax CN column using MeOH : HaO : NH40H as mobile phase and UV (282nm) as detector. The distribution of these alkoloids in the genus of Stephania were discussed.