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期 刊 ：植物遗传资源学报 2015年 16卷 3期

关键词：春兰；品种；AELP；遗传多样性；

Keywords:Cybidium goeringii, Cultivars, AFLP, Genetic diversity,

摘 要 ：为了揭示春兰品种的遗传多样性和亲缘关系，为春兰种质资源的有效利用和开发提供依据，采用AFLP技术对51个春兰品种进行了遗传多样性分析，经筛选得到了8对条带清晰、多态性高的引物，共扩增出1315条DNA片段，其中多态性条带为1217条，平均1对引物扩增条带164条，多态性带152条，多态性位点频率为92.5%，表明春兰品种具有丰富的遗传多态性。49个品种含有特有带。51个品种间遗传相似系数变化范围为0.501~0.716，聚类分析表明，51春兰个品种共分为5个类群，来自同一地区的品种并没有聚在一起，表明春兰品种的遗传背景混乱。AFLP分子标记技术能有效地分析春兰品种的遗传多样性和亲缘关系。

Abstract:The genetic diversity of 51 Cymbidium goeringii cultivars was evaluated based on AFLP molecular markers in this study. Among the 1315 DNA bands obtained from eight selective primer pairs，1217 (92.5% ) were polymorphic. The average number of DNA bands per primer pair was 164, and the number of DNA polymorphic bands per primer pair was 152. It was represented that 51 Cybidium goeringii cultivars had abundantly genetic diversity. 49 cultivars had their distinctive bands which could distinguish one cultivars from the others. Genetic similarity coefficients among the cultivars ranged from 0.501 to 0.716. Cluster analysis indicated that the 51 cultivars could be divided into five groups. The cultivars which collected from the same place were not clustered to the same group. It indicate that domestication causes confusion in the genetic backgrounds of C．goeringii．AFLP can be effectively applied to study the genetic diversity and relationship analysis of C．goeringii．

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