Abstract:Shikonin production of Onosma paniculatum Bur. et Fr. callus was inhibited by combination of 2,4-D and NAA of lO-5mol/ 1 and lO-6mol/ 1 with lO-5mol/ 1 KT separately, and was not affected by combination of IAA with KT in concentration given. Glu-cose was more effective on shikonin production in callus than sucrose. An appropriate concen-tration of glucose and sucrose on shikonin production was 6 separately. Lactalbumin hydrolysate (LH) and casein hydrolysate (CH) inhibited production of shikonin in callus. When concentration of CH was bigger than 0.02, the growth of callus was inhibited. LH had no obvious effects on callus growth. Coconut milk-which concentration was 10 stimulated production of shikonin which content was 24 times of the control.