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作 者 ：王钧 胡运乾 李英 张纯

期 刊 ：云南植物研究 1983年 5卷 04期 页码：1-3

关键词：冠瘿；Nopaline合酶；不同；Nopaline合酶；定量比较；

Keywords:quantitative, nopaline, synthase,

摘 要 ：本文发展了一种根据NADPH消耗定量测定冠瘿组织粗酶提取液中nopaline合酶活性的方法。对于含nopaline型Ti质粒的土壤根癌杆菌菌株透发的马铃薯、向日葵、烟草和胡萝卜冠瘿系来说，由于不存在酶反应的促进剂或抑制剂，粗酶提取液中nopaline合酶的活性能够反映体内存在的此酶的酶量。用这个方法测定了四株土壤根癌杆菌菌株诱发的十六株植物冠瘿系的 nopaline合酶活性，由不同菌株诱发的所有胡萝卜，烟草冠瘿素包括单细胞克隆的烟草冠瘿系，以 DNA为单位的此酶量明显地低于向日葵和马铃薯冠瘿系。由此推测，不同宿主植物中nopaline合酶量的差异很大程度上取决于宿主植物本身。通过Ti质粒把外源基因导人不同植物可能有不同的命运。

Abstract:A quantitative method for determination of the activity of nopaline synthase in the crude enzyme extract of crown gall by measurement of the expense of NADPH was established. The activity of this enzyme in vitro for the crown gall lines of potato, sunflower, tobacco and carrot induced by Agrobacterium tumefaciens carrying nopaline type Ti plasmid can represent its quantity in vivo for no any activitor or inhibitor of this enzyme for sixteen plant crown gall lines induced by four A.tumefaciens strains was measured by this rnethod.The quantities of this enzyme in all crown gall lines of carrot and tobacco including one of the single cell line of tobacco crown gall on the basis of DNA unit are ess viously than the quantity of it in all crown gall lines of sunflower and otato induccd by simillar A. tumefaciens strains.The different quantity of no- aline synthase in different host plants is determined to a great extend by the ifferent plants thomselves. It is possiblat that a cxterior gene incorporated into plant though Ti plasmid wi11 get different results in different plants.

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