免费文献传递   相关文献

Study on transport characteristics of honokiol in Caco-2 cell model by HPLC

HPLC考察和厚朴酚在Caco-2细胞模型的转运特征



全 文 :.8C
‘KÑ_`Q]
$G/abcâ[Kd¯e
¶V
P# W
#
÷Ž;
P# W
#
\‚}
P
#
~Ëf
P# W
!
!Ph
ãüãV8HKA¯ 8HKýþëjLMJ
#
ãã ãü
LWONMN%
Wh
ãV8HKA¯ @KSÿLM8!
#
ãã ãV
LPMMMX
$
‚;
%!

&
ƒ„Ñ_`Q]
$G/abcâr[KdÆÜ

•–
. D%/I$0&PMMPN
{m”
$^hL IIiWLM II! L
!
I%!
1Êj“e
<
¸
$[MsOM%!
W%Áx
WMO )I

$G/abc⃄Ñ_`Q[ð†Kd
!
‘K7š
#
ÇÌ<
#
¤ÜÛ
#
U.#
@AÑ_`QKd[TU


.8C
W%ÇÌ<
!
.Pª¼J<ÿF(

—˜
.
Ñ_`QÀ+=nê
OYW^ M^^
SQO [XOhX $:YMh___ N%!
W%#
MhM^
!
I/&/C
QP

Ñ_`Q]
$G/abcâr
!
±ab4>ü9¥ü[Kd·6¥ü9
>ü[Kd
!
]jZ<ïqªÑ_`Q]
>8
#
78
Ñ
78
#
>8
…†[Kd·¬7šSºx23
&
íc?‡*u¡µ#Ñ_`
Q
>8
#
7C
[Kd·
&U.
AÑ_`Q]
>8
#
7C
…†[KdTUY.u
&
Ñ_`Q]
>8
#
7C
Ñ
7C
#
>8
…†A@è¦@›

—™
.
Ñ_`Q]
$G/abcâr[Kd‚&ê<ÊKd
!
Aè`þ}M[‚ÊKd
!
Z76K9
8<*U
IÝt[®E›

$
ƒ„…
%!
Ñ_`Q
&$G/¼J<ÿF(
&8<*U
IÝt
&
<ÊKd
$
8†$f
%!WMPMPPWPMM[
$
‡ˆEq
%!
­æ
)
ÿ|È
*
ö÷Ýu.vuv
$WMMX7>;PP7MN$
‰€>Š
%!
!
@6@
!
ƒ„Y
!
ƒ„…†rNJǃ„
!A(&.$MWM%
O_OLNML^!-$
>Šš›
%!
úâ
!
z{¾
!
ƒ„…†rNJÇiÇôÊN]
!A(&.
$MWM%O_OLNPMO!-!!
_`Eê›rÇ
!
wè|7n›
#
ª§äf‡
Þé
!
›7Bir
#
CDÑØ
#
vU§B
#
[EÉ
F
#
›Gp
P(

Ñ_`Q
$ #/)/V/&%
N±rÇ_
`r64µ=[ZèQŽs¨\ÄÌ
!
Ç¥ƒ„¼
.Ñ_`QwèXÈQ¨
#

#
m3
#
¤ÜQ6«
q®ÑmßëLJ™ E›
W(

Ȩ̂¨ƒ„
¼.
!
Ñ_`QqrÑ?´ÛÛÇ[ã̬›B

!
âèe·ÈâÇÌ[GH
O<^(

­~®”
WM
÷
;Ï6•D›
$G/I$G(&&)(0%
abcâEêþ®câ;îÉÇÌG
Hƒ„
L(
!
4câiÇÌ]þ~GHèo![j‰
›

‰Ñ_`Q]
$G/abcâr[GHÆÜ
]­~®7«r¶Ã1‘

¤§¨D›
$G/ab
c⃄Ñ_`Q[GHÆÜ
!
B†7š
#
ÇÌ<
#
¤ÜÛ
#U.#
@AÑ_`QKd[TU
!
•m¡
ižþ§¨()j0Ä
!
‰.Ñ_`Qã̬›ê
[ÈY
!
êÛâ¾îµo¥,¦)

!
ÁÂ
s›H
$OL C!C1[LM%&,XMM
â.}²@¸0
$
_&|²ö]ßàè#–$
%&RWWLP
.N8@
$PZPM

% $
6çS»|ö÷è#–$
%&TT
W
õ=0
$
øùÈãú…ö÷ïè#–$
%&
..1WPhWL
.}²@¸dI
$
_&XI]ß
àå
%&1c<9ð±HTì\NEò
$
]çìáÝè#–$
%&T&BIU40;+W<18
J£uXv
$
¥
¤
%&A@C#Lò}4ßÆ
$
_&òKö]ßà
å
%&1eP²@LNMNà
$
_&$»ßàè#–
$
%&
 ÙÁó\Û
$
6çSÎý ÙáÝè#–
$
%&a+¨µ}WOn¼à
$
ÀœPÀ…äá
Ýå
%&R.@<_P^M>
.}²@QbÕ|0
$
_&š+
ö]ßàè#–$
%&C
#ésj{mß
$
¥
¤.}
%&
ab.Rß
$=&G(&<-31!
Ù­
%
$G/aba
$
röè_&abÍ
!
ab²!
ô(ê
^M gLM
ô
%&
Ñ_`Q
$
r­ÇFãÌÜF
WÔ²
!
Ãf
PPM[OMŒŽ
#
“eê{m=
$
{­S:–$
%&
¸ê =¸
&PW
 
A%)$F0(&
õ=
«
#[L GI
W
õ=í
$/%))*–$
!$Id%5*(!
=>! ,1>%&R=-=
õ=s
$R4&d(GG/t1 =/56&(5 -$<
*&(10I(54I!@dG/%&
TÏQó
$J(2$&d/E)(0(%<
4I!.BG&/)(%&
5‘וs@
$ )/)(00()2$&$I)/
$G50!?->>!.BG&/)(%& =<
6•U
$ =<*&42$I)(!
1*I$%&
çV—
<
ÂV—ðms
$ 1*I$%&
WÝt®
$2%BU0)!1*I$%
#
…”
# !^
GFáÜ
/XNWP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
# !^ !^
Ñ_`QXÝs
!
93P#Ñ_`QA“
F2·
!
aŒŽñlÔ±
P IC!
áÜÚ
WM II/&/
C
QP
[Ñ_`QXÝs

# !^ #^Pm
q­“”óYIŽZHž@•
$
Ñ@
!
93G#Ñ@
±
PMM IC
·ír
!
a2·
U.[h^
[
.$%Vf0
@Ùæñs
$.711%
a} Ùñl
!
›
U.[h^
[
.711
Ô±[
!
þU6(ê
Pm
# !^ $^
íc?‡ñs
!
93P#íc?‡2·
!
a
ŒŽñlÔ±
PM IC!
áÜÚ
WM II/&/C
QP
íc
?‡ñs
!
93G#íc?‡ŒŽñs2·
!
›
U.
[h^
[
.711
Ô±
LM IC
·ír
!
éY<ê
WMM
!
I/&/C
QP
!
Eêíc?‡XÝs

# !^ %^
Ñ_`Q
.711
r[GF
!
93G#Ñ
_`QXÝs2·
PM IC
·ír
!

P ICPm
Ñ@

U.[h^
[
.711
Ô±[
!
áÜÚ
PM!WM!LM!NM!PMM!PLM!WMM!OMM!^MM
!
I/&/C
QP
[
GF
!
ŒŽéYWm!
Ñ@
[Z·ê
MhPm
# !^ &^
Zíc?‡[GF
!
93G#
L IC
íc?
‡XÝs
!P ICPm
Ñ@
!
C
Ñ_`QXÝs

PM IC
·ír
!
a
U.[h^
[
.711
Ô±
PM IC
# !^ ^
YZ
U.
[Ñ_`Qñs
!
V
Pm
Ñ@
ñsÑ
U.[h^
[
.711
ñs›
.&!?$T.
ñs6
ŒAc
U.
ê
L!_!
’
# !^ %^
u[GFáܔáÜY
Z
U.
[GF

# #^
ab¼›ç¨
V
$G/abbs† U3Ö¥[
_X
 «
r
!
7 a
PMM
!
C!
£
O[ j! LmT
W
õ=0r
õ=
!
é¾aÐ
# !^
uª[
_
f<[Ñ_`
Q
.711
GFñs
!
>áA“ 
!
\b
^ #
¾GKñ
saÐ
=AA!
Öõ=
^ #!
GV_ós
!
aÐüŒs
.]
$R=1T%!
›®Àß%Ô7 _

_%0•
!
&9ab[òO<

# $^
ð†Kd§¨
¬›§¨`õ-[
$G/abcâ
X(
!
Ô
A%<
$)0F(&
«õ=±{
WP
8
!
¿tñsä3¾
!
›³}
[
8.[h^
[
.711
\ãxè
$G/[
A%)$0F(&
«
!
Öaг}[
.711!O[ j
ïqªõ=
WM I)!
Gb
.711!
Öaг}[
.711!O[ j!LmT
W
ïq
ªõ=
WM I)!
Gb
.711 >8
09
7C
0[Kd
.
V
MhL IC
GFa9
>8
0Eêo۝
!
Z7]
7C
0aÐ
PhL IC
ct
.711
Eê†K
&
±
7C
09
>8
0Kd
.
V
PhL IC
GFa9
7C
0Eêo۝
!
Z7]
>8
0aÐ
MhL IC
ct
.711
Eê†K
!
U
A%$)0F(&
õ=«£
O[ j!LmT
W
[õ=0r
!
6Œ]
OM!XM!_M!PWM I)
G#†H`[Kds
MhL
IC$>8
#
7C%!MhWL IC$7C
#
>8%!
Z7‚aj\[
ct
.711
H®GF€&ݛ

# %^
6‹…”[õ-
# %^ !^
Ñ_`Q[6‹…”[õ-
!D%/I$0&
PMMPN
{m”
$^hL IIiWLM II! L
!
I%!
1Êj
“e
<
¸
$[MsOM%!
1L
P IC/I)
QP
!
”@
WL j!
W
%Áx
WMO )I
# %^ #^
W%Áx[´µ
!,
r­ÇÒ
-
rÑ_`Q
[W%Áxê
W_^ )I!
-¤§¨GF·[#Ü
!
Y#AW%#µK0#[&
!
§¨0•¼.Ñ
_`Q]
WMO )I
[W%#ê
MhM^
!
I/&/C
QP
!
]
W_^ )I
[W%nê
MhW
!
I/&/C
QP
!
Y#´µ
WMO
)I
Eꤧ¨[W%Áx

# %^ $^
GFÖ¥
!
#GFñs
N MMM %/I)
QP

PM I)!
G#_ós
!
îG6‹

# %^ %^
À+=n[õ-
!
93·#XÝs2·
!
’
# !^ $^
uGFá܅”áÚ=><ê
WMM!PMM!
NM!^M!WM!W
!
I/&/C
QP
[FJA“Fñs
!
GFñ

# %^ $^
GFÖ¥u^E¾îG

À+=nê
OYW^ M^^SQO [XOhX $:YMh___ N% .8C
{md
Ãd
P
# %^ &^
93
#
ŸÔ›ç¨
!
¤§¨‘KÑ_`
Q
WMM!PMM!LM
!
I/&/C
QP
O
YZ<[93Ñ
ŸÔ›
!
93
31R
6ΐ
Ph[m!MhWMm!Ph^m&
ŸÔ›
31R
6ΐ
MhWLm!MhPMm!Mh^Pm!
¼.#
…”2ĝÑ_`Q[Z·%Ô

# &^?
$UU
ÇÌÿ¡
$G/abH4[¼JlÿF(›
?
$UU
Eꐑ
!
’ª}ÇÌ.PKds
.
?
$UU
Y5UZ$5!/5/2
Q
%GIZ0
}r
5U$)I/&%
ê
5!$0%
~[Kd·
!5$GI
W
%
ê
XTU
!2
/
$)I/&/C
QP
%
ê]
$G/H4ab[¥
0Jæ0[ÇÌÄ<

=0KdsH_
?
%$2/
’ª}.P
.
?
%$2/
Y?
$UU$7C
#
>8%
Z?
$UU$>8
#
7C%
}r
?
$UU$7C
#
>8%
Ñ
?
$UU$>8
#
7C%
6Œêð†¼J<
ÿF(


=G%/0/6(GKG(&WMMO
îÉ()Ö¥

/[NWP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
dP!
ctKds
$>%#
Ñ_`QÀ+F
$7%#
Ñ_`QKds
$%
[
.8C
d
$

$ !^
7šGYZ<[Ñ_`Qñs]
$G/abcâ

>8
09
7C
0i±
7C
09
>8
0[Kd·¬
7š[D\Ãd
W!O
d
W!
7šAÑ_`QKd[TU
$>8
09
7C
0
%
d
O!
7šAÑ_`QKd[TU
$7C
09
>8
0
%
]jZ<ïqª
!
Ñ_`Q[Kd·Sºx
23
&
Ñ_`Q±
>8
09
7C
0[KdLs¬<
[º#faT
!
h.ªKdLs‚&K!
Ã
d
^&
Ñ_`Q
>8
#
7C
[Kd·&|
78
T9
>8
T[Kd·
!
h.Ñ_`Q[Kdè|Ô[…†
›
!
Ãd
L&
Ñ_`Q[
?
$UU$7C
#
>8%
i
?
$UU$>8
#
7C%

[H_
?
%$2/
]YZ<ïqªŒAH]
MhL gP!
Ã
¼
P
_£0•¼.Ñ_`Q]
>8
#
7C
…†[K
d…}ê‚&ê<ÊKd
!
Z7Aè`þ}M[‚
ÊKd

d
^!
YZ<[Ñ_`Q[KdLs
$>8
09
7C
0
%
d
L!
YZ<ïqªÑ_`Q
?
$UU
D$

Kp9!(YO%
¼
P!
YZ<[Ñ_`Qñs[
?
$UU
_
$

Kp9!(YO%
Ñ_`Q
Z
!
I/&/C
QP
?
$UU
ZiPM
Q[
GI/0
QP
>8
#
7C 7C
#
>8
?
%$2/$7C
#
>8Z>8
#
7C%
LM ^:W[ pM:PW O:W^ pM:PO M:[X
PMM O:OX pM:W^ W:X[ pM:PO M:[_
WMM W:^O pM:PW P:_P pM:MW M:[_
$ #^
¤ÜÛíc?‡AÑ_`QKd[TU
íc?‡*u¡µ#Ñ_`Q
>8
#
7C
[
Kd·
!
ìNA
7C
#
>8
Pèu¡[TU
!
¼.
Ñ_`Q]
>8
0K9
8<*U
Ýt[®E›
!
Ã
d
X!
¼
W
/NNWP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
dX!
íc?‡AÑ_`QKd[TU
¼
W!
íc?‡AÑ_`QKd[
?
$UU
_[TU
$

Kp9!(YO%
GF
?
$UU
ZiPM
Q[
GI/0
QP
>8
#
7C 7C
#
>8
?
%$2/$7C
#
>8Z>8
#
7C%
Ñ_`Q
!!!!! O:OX pM:W^ W:X[ pM:PO M:[_
íc?‡
n
Ñ_`Q
L:P^ pM:WX W:^L pM:LX M:^N
$ $^
@AÑ_`QKd[TU
@]ÇÌ[6XKdr³Ci&[E›
!
ª
TUabX_[KdÝt[כ
!
AÇÌ]
$G/abX_[Kdäãi&[TU

]
^ j
ª
!
Ñ_
`Q]
XM I)
7]
>8
#
7C
Ñ
7C
#
>8
…†[Kd
LsOPªð
!
]
PWM I)
¾ªKd·ðê
W gO
ý
!
Ãd
[!
¼
O
d
[!
@AÑ_`Q6XKd[TU
¼
O!
YZ@ªÑ_`QKd[
?
$UU
$

Kp9!(YO%
@
Zj
?
$UU
ZiPM
Q[
GI/0
QP
>8
#
7C 7C
#
>8
?
%$2/$7C
#
>8Z>8
#
7C%
O[ O:P^ pM:M_ P:O^ pM:MP M:[M
^ P:O^ pM:MP W:WP pM:MO M:L[
$ %^U.
AÑ_`QKd[TU
8.
AÇÌ[GHèi&[TU
!
*3[
U.
ÑÇÌlW›YTUª]
$G/abX_[Kd
!
Ä2[
U.
躝ÇÌ[GH

Ñ_`Q]B{
[
.711
r[Kd·I™
!
ì5þ;h
U.
AÑ_`
Q[KdPèu¡[TU
!
Ãd
N!
¼
^
d
N!8.
AÑ_`Q6XKd9TU
$>8
09
7C
0
%
¼
^!
YZ
U.
ªÑ_`Q[
?
$UU$>8
#
7C%
$

Kp9!(YO%
U.
?
$UU
ZiPM
Q[
GI/0
QP
L Wh__ pMhPM
[h^ OhP^ pMhM_
_ OhWM pMhPW
%
†,
/G/ab;¬±¨0Áab
!
ª01Ñ
ã\¯-¨5±¨6Œab
!
Zèi6~ë
Œj‰[®
!
gô]ab¸@µo‰ÇÌ6N
m¡6)X[GH
#
ôƒ
#
Kdn¢
[(

§1_
!
]
2%$)0F(&
Fãr
!
Kd¡ôr[
W
…†[<
f¾D>YNOA‡[
!
§¨r
>8
#
7C
[
?
$UU
_&|
7C
#
>8!
8G[CŒN-
$G/a
bH4
>8
06\KvP
!
ª_`5TU||#
7C
0TU
!
8Í&
$G/abH4
>8
0wèû|
[GHTU

8GMí
>8
#
7C
[
?
$UU
B|
!
±d
*¦KÑ_`Q[KdLs¬<[º|PèK
R¡aÑ[Rå
!
h.ª*gNKÊX2

Z7Aè`þ}M[‚ÊKd

íc?‡
[aÐ*u¡µ#Ñ_`Q
>8
#
7C
[Kd·
!
h.ªKdK
8<*U
Ýt[®E›

@AÇÌ[Kd[TUN0j£[
N(
!
wx
abX_[`þ[כ
#
ab[¤W[ãxכ
#
a
bgš[šô
#>8
0[vP[dÊ[T¢GÇÌ
[ñl›‡BK9@[TU
!
ê@ä͏_[Y
—KRY¬ÇÌ[Kd

Ñ_`Q]
^ j
ª[K
d·jA
O[ j
&ê0™

0™ÇÌA
U.
è0|
[¦@›
!
f¤7r
U.
AÑ_`Q]
$G/[T
UYN0|

‹™ƒ„¼.
_!
abcâižþGHèo
![j‰›

Nƒ„ÇÌGHÆÜ
!
³%qrÇÌ
[þ~GH
#
ã̬›GòO›
!
ŠÇÛâá.[
o!Nw

ìN
!
ab;¬0
!
…a6b)s[
cëab
!
Yf…a6_Œ[)s4
PP(
#
‡…a
/_NWP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
‘[dʇY—AÇÌGH[TU

²
!
§¨
0•6×î|ÅižþȨ̂¨()j0Ä
!
êŠ
Ûâ[á.µo¥,¦)

$
‹ŒŽ
%
P (!
r­ÇÒ
:

1(:P__L. WPN:
W (!8B/=D! C((a! a4)<#/.1:>)20224()200/&$2(5 6%/I2#(d$%V0$)5 6%420/6#73().7 )>)F7!7
9(:>%G# 8#$%I3(0! WMMW! WL $O%. OWL:
O (!

!
×%e
!
úv
:
_`QiÑ_`Q]|}þ~[Çô
ÊN]
9(:
l^tÇ]]1
!WMMO! P_$^%. WLN:
^ (!
)fã
!
̲9
:
_`ûÛÇ[ƒ„
9(:
r­rÇ£¤
!
P__L! WM$P%. OM:
L (!a$)*.A! c$)*@9:A%$)0U/%2$)5 4U2$V(G#$%$G2(%02(0/6$
)(F5(%E$2E(/6d(%d(%)($8,(U2#(&$&G(&&)(. $G/G2$8#$%I$G/&1)! WMMO! W^.
PPNL:
X (!
úâ
!
þìg
!
$â­
:$G/abcâ[õ-Gª]rÇG
Hƒ„r\›B†
9(:
rNJÇiÞßÇ¥
! WMPM! WP
$X%.L[Mh
[ (!
‰h
!
$i
!

:$G/abcâ
<
ÇÌGHƒ„[èé
)
Nw
*9(:
r­Ç¥]m1
! WMM^! WM. XM_:
N (!e#4 e7!=$V#H$1 D!C4 7!(2$&:A%$)0U/%2$G%/00$U/&$%b(5
I/)/&$B(%/6$G/E%402%$)0GB2/009(:S%/&/*B! WMM^! OWL. PPX:
_ (!.&*(%0>3! 1I2# R8! 7(%I$G#(%99! (2$&:8%(5G2)*/%$&
$d0/%U2/) /65%4*0. $G$0(0245BF2# $)/E(&G&$00/6$)2IG%/<
d$&$*()209(:8#$%I3(0! WMMO! WM. PP^_:
PM(!>%24%00/) 8! 7/%G#$%523A:;)2(02)$&5%4*$d0/%U2/) $)5 I(<
2$d/&0I) G(&G4&24%(0. $G/P__[! P^.PXLL:
PP(!D$%&00/) 9! cVI$) >!>%24%00/) 8:A#(I4G40&$B(%$0$d$%<
(%2/5%4*$d0/%U2/) ) I/)/&$B(%06#4I$) )2(02)$&(U2#(&$&
.AW_<.*/d&(2G(&09(:;)298#$%I! P__O! __. WM_:
=DIHF BCDG/CKMBGDTE/G/TDAG0KD0TKBLEBCBY0B10C2/TB-W TA1.BHA1NF 3O;2
e-?@7$/
P!W
! c,>)*4/
P!W
! .-?9$))$)
P
! C>;+$U)*
P!W
!
$Ph%1917:6, C(9!!/!1)8#7!,107!67.<(3(11:(3 )8$/7(3J,)/ E(F1:9!-)82,(191
#1+6(1( P)(33/7(! P)(33/7( LWONMN! 2,(7&
Wh%1917:6, 21(!1:)8;1NP:/3 P1F1.)*01(!! $/7(3J,)/ E(F1:9!-)8
2,(191#1+6(1! $/7(3J,)/ LPMMMX! 2,(7 %
6NKDG/TD(8N]ATD0RA. A/0245B2#(2%$)0U/%2I(G#$)0I/6#/)/V/&) $G/ADEBH. A#($)$&B00F$0U(%<
6/%I(5 /) $D%/I$0&PMMPN
G/&4I) $^hL IIiWLM II! L
!
I% (&42(5 F2# $G(2/)2%&(2(G2/) F$E(&()*2# F$00(2$2WMO )IhAF/5%4*G/)G()2%$2/)! )#d2/%! U.! 2(IU(%$24%(/) 2#(2%$)0U/%2/6#/)/V/&F$0)E(02*$2(5hA#(5%4*G/)G()2%$2/)0F(%(5(2(%I)(5
dB#*# U(%6/%I$)G(&]45 G#%/I$2/*%$U#B$.8C% $)5 40(5 2/G$&G4&$2(2#($UU$%()2U(%I($d&2BG/(6G()2h:AKI1D. A#(02$)5$%5
G4%E(/6#/)/V/&F$0OYW^ M^^SQO [XOhX $:YMh___ N%! $)5 2#(5(2(G2/) &I2F$0MhM^
!
I/&/C
QP
h;) $G/2%$)0U/%2$I/4)206%/I2#(2/U 05(2/2#(d$0(05(/6F(%(I/%(2#$) 2#$26%/I2#(d$0(05(2/2#(2/U 05(4)5(%2#(0$I(G/)G()2%$<
2/)hA#(2%$)0U/%2$I/4)20)G%($0(5 F2# 2I(d/2# ) >8
#
7C$)5 7C
#
>85%(G2/)0hS(%$U$I&G/4&5 IU%/E(2#(2%$)0U/%2
$I/4)20/6>8
#
7ChA#(%(F(%()/(6(G20/6U./) 2#(2%$)0U/%2/6>8
#
7Ch7/2# ) >8
#
7C$)5 7C
#
>85%(G2/)0! 2#(2%$)0U/%2
0#/F(5 2(IU(%$24%(5(U()5()G(h2BCT1IK0BC. ./)/V/&02%$)0U/%2(5 2#%/4*# 2#()2(02)$&I4G/0$E$$U$00E(5640/) I(G#$)0I
U%I$%&B! G/(K02)*F2# $G$%(%aAF WBGHK(!#/)/V/&& $G/HB0.!+b%#)QT]T..#+!!!++(
$
$Q
!
žŸ 
%
/M_WP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP