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作 者 ：黄林芳

期 刊 ：中国中药杂志 2011年 36卷 5期 页码：.

关键词：太白贝母；水溶性成分；HPLC-DAD；质量控制；

Keywords:Fritillaria taipaiensis, Aqueous Extracts, HPLC-DAD, Quality control,

摘 要 ：目的：建立应用RP-HPLC-DAD 同时测定太白贝母药材中7 种核苷类活性成分（胞苷、尿苷、鸟苷、胸苷、腺苷）及碱基（尿嘧啶、腺嘌呤）含量的方法。方法：采用Zorbax SB-Aq C18 色谱柱（4.6 × 250 mm，5 μm），流动相为甲醇和水，梯度洗脱，流速1 mL•min-1，检测波长260 nm，柱温为25℃。结果：尿嘧啶、胞苷、尿苷、鸟苷、胸苷、腺苷、腺嘌呤的质量分别在0.312~ 37.440，1.095 ~ 65.700，1.020 ~ 61.200，1.075 ~ 64.500，0.812 ~ 48.720，1.070 ~ 64.200，0.432 ~ 25.920 g•mL-1 浓度内呈良好线性关系。平均回收率为96.96％～103.52 %，RSD < 3.80 %。，符合分析要求。结论：太白贝母有紫外吸收的水溶性成分主要是核苷类成分，所建立方法简单、重复性好，准确可靠，可作为太白贝母药材及相关制剂的质量控制方法。

Abstract:Objective: To develope a high performance liquid chromatography coupled with a photodiode array detector (HPLC-DAD) method for simultaneous determination of 7 nucleosides and nucleobases in Fritillaria taipaiensis. Method: The analyses were performed on An Agilent Zorbax-SB-Aq -C18（4.6 × 250 mm，5 µm）was used for all analyses. The investigated compounds were separated with gradient mobile phase consisting of water and Methanol. The flow rate was 1.0 mL•min -1; the detection wavelength was 260 nm. The temperature of sample manager was set at 25℃ and the injection volume was 20μL. Result: The investigated compounds including uracil, cytidine uridine guanosine, thymidine, adenosine, and adenine were shown good linearity (r2 ≥0. 999) over the tested ranges. The average recovery was 96.96 ％～103.52 %with RSD ≤ 3.80 %. Conclusion: The accuracy, stability, repeatability and average recovery of the method are satisfying, and the seven nucleosides and nucleobases components of F. taipaiensis can be rapidly and accurately quantified by HPLC-DAD. This work provided helpful information for comprehensive quality evaluation of F. taipaiensis.

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