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作 者 ：徐静,孙长凯,马辉,王禄,张健,张玉梅,吴兰香

期 刊 ：中国中药杂志 2010年 35卷 1期 页码：114.

关键词：银杏叶提取物；银杏内酯B；谷氨酸；海马神经元；

Keywords:EGb761, GB, glutamate, hippocampal neuron,

摘 要 ：目的: 经预处理给药模式观察了银杏叶提取物(extract of Ginkgo biloba, EGb761)及其单体银杏内酯B(ginkgolide B, GB)对神经元损伤的影响,以探讨EGb761及GB在抗兴奋毒性神经损害中的应用价值与实施途径。 方法 :原代培养新生Sprague-Dawley(SD)大鼠的海马神经元,建立谷氨酸诱导的兴奋毒性模型。采用台盼蓝染色、凋亡神经元测定及乳酸脱氢酶(LDH)测定的方法观察不同剂量EGb761,GB 的神经保护作用,并与NMDA(N-甲基-D-天门冬氨酸盐)受体非竞争性拮抗剂MK801的神经保护作用相比较。 结果 :EGb761,GB在不同剂量下均能不同程度的提高细胞存活率,降低凋亡率,减少LDH泄露,且在一定范围内保护作用呈剂量依赖的方式。EGb761,GB分别在100 mg·L^-1,100 μmol·L^-1的剂量下有最佳的保护效果,但均弱于MK-801(10 μmol·L^-1),GB最佳剂量下保护效果优于EGb761。 结论 EGb761,GB对谷氨酸兴奋毒性损害有保护作用。在预处理给药模式的最佳剂量下,GB的保护效果优于EGb761,将其用于高危人群的预防干预可能有更大价值。

Abstract:Objective : To observe the effect of the extract of Ginkgo biloba (EGb761) and the components isolated from the extract named ginkgolide B (GB) against damage of glutamate in pretreatment modes so that determine their application value and approach. Method : Based on glutamate-induced excitotoxicity to primary cultures from neonatal Sprague-Dawley(SD) rat hippocampal neuron, our experiment utilized trypan blue, TUNEL and LDH to study the effect of EGb761and GB on neuron in different doses pretreatment modes, as well as to compare with the NMDA receptor uncompetitive antagonist-MK-801. Result : EGb761 and GB can recrease cell viability, reduce apoptosis rate and decrease LDH leakage in different degree and depended on dose in certain range. The maximal protection was achieved at a concentration of 100 mg·L^-1, 100 μmol·L^-1, but inferior to MK-801 (10 μmol·L^-1). The protective effect of GB is superior to EGb761. Conclusion : Treatment with EGb761 and GB could protect the neurons against glutamate-induced injury. The maximal protection of GB was achieved by pretreatment is superior to EGb761, so its precautionanary intervention to high-risk population could have more value.

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