Abstract:Objective: To study the chemical constituents of Stellaria media. Method: The compounds were isolated with various column chromatography and semi-preparative HPLC. The structures were determined by modern spectroscopic methods (IR, UV, 1H-NMR, 13C-NMR, HMBC, ESI-MS). Result: Five compounds were isolated and identified as apigenin 6-C-β-D-galactopyranosyl-8-C-α-L-arabinopyranoside (1), apigenin 6-C-α-L-arabinopyranosyl-8-C-β-D-galactopyranoside (2), apigenin 6-C-β-D-galactopyranosyl-8-C-β-L-arabinopyranoside (3), apigenin 6-C-β-D-glucopyranosyl-8-C-β-D-galactopyranoside (4), apigenin 6, 8-di-C-α-L-arabinopyranoside (5). Conclusion: Compounds 1-5 were obtained from Stellaria genus for the first time.