---

期 刊 ：中国中药杂志 2007年 32卷 10期 页码：899.

关键词：三叶木通；愈伤组织；诱导；

Keywords:Akebia trifoliata, callus, induction,

摘 要 ：目的：探讨影响三叶木通愈伤组织诱导因素,建立三叶木通愈伤组织培养方法。方法：采用组织培养方法,比较不同外植体、培养基、pH、温度、光照、植物生长物质种类及其配比对三叶木通愈伤组织诱导的影响。结果：叶片的诱导率最高,为87.5%；茎段次之,叶柄较差；培养基MS,B5愈伤组织诱导率较高,达到了80%以上,高于培养基H,SH和改良White的愈伤组织诱导率；偏低的pH对愈伤组织生长有利,出愈率高,但从愈伤组织生长质量方面看,不同pH差异不明显；愈伤组织诱导最适温度为25 ℃。结论：三叶木通愈伤组织的适宜培养基为MS+2,4-D 4.0 mg·L^-1+NAA 1.0 mg·L^-1+KT 1.0 mg·L^-1(pH 5.8),培养温度为25 ℃,暗培养。

Abstract:Objective: To investigate the inductive factors of effect on Akebia trifoliata and establish culture method for A. trifoliata callus. Method: To study the possible effective factors of culture condition by comparing with different explantation, nutrient medium,pH, temperature, illumination, growth substance of plant and its ratio. Result: The inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B₅ callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 ℃in temperature. Conclusion: The best culture condition for callus was the leaves as explantation. The A. trifoliata callus culture’s best inductive condition was MS+2, 4-D 4.0 mg·L^-1+NAA 1.0 mg·L^-1+KT 1.0 mg·L^-1 (pH 5.8), cultural temperature was 25 ℃, cultivation was dark.

---