---

期 刊 ：中国中药杂志 2007年 32卷 08期 页码：706.

关键词：雷公藤；超临界萃取；指纹图谱；

Keywords:Tripterygium wilfordii, supercritical fluid extraction, fingerprint chromatography,

摘 要 ：目的：建立雷公藤超临界CO₂萃取物的色谱指纹图谱。方法：采用高效液相色谱法对其进行测定,色谱柱：Kromasil C₁₈(4.6 mm×250 mm,5 μm),流动相乙腈-1‰磷酸水溶液进行梯度洗脱,柱温35 ℃,检测波长267 nm,分析时间80 min；流速1.0 mL·min^-1,测定10批超临界萃取物的色谱图,确立标准指纹图谱。结果：由此指纹图谱标示出雷公藤超临界CO2萃取物的27个共有峰,以化学对照品结合HPLC-MS鉴定了其中21峰。结论：该分析方法的稳定性、精密度、重现性较好,为雷公藤有效部位的质量控制提供了依据。

Abstract:Objictive: To establish the chromatographic fingerprint of supercritical carbon dioxide extract of Tripterygium wilfordii. Method: HPLC method was applied for quality assessment of T. Wilfordii, HPLC analysis was performed on Kromasil C₁₈(4.6 mm×250 mm, 5 μm)with the mixture of acetonitrile-1‰H₃PO₄, as mobile phase in gradient mode. The samples were detected at UV of 267 nm with column temperature of 35 ℃, analytic time was 80 min; Flow-rate was 1.0 mL·min^-1. The chromatographic fingerprint of ten batches of samples was determined, for establishing the chromatographic fingerprint of T. Wilfordii. Result: Indicating 27 peaks in common, identified 21 peaks with chemical reference and HPLC-MS, and the HPLC fingerprint was established. Conclusion: The method is steady and accurate with a good repeatability and can be used as a quality control method for T. Wilfordii.

---