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期 刊 ：中国中药杂志 2008年 33卷 14期 页码：1739.

关键词：桦木酮酸；肿瘤细胞；细胞周期；P53；PI3K；AKT；

Keywords:betulonic acid, cell cyle, P53, PI3K, AKT,

摘 要 ：目的：研究桦木酮酸对H22荷瘤小鼠肿瘤细胞周期及相关蛋白表达的影响。方法：采用流式细胞仪检测H22肿瘤细胞细胞周期的变化以及P53蛋白的表达变化；免疫组化法检测H22细胞中的PI3K和AKT蛋白。结果：随桦木酮酸剂量的增加，H22细胞S期和G₂期的含量逐渐增多且P53蛋白的表达随之升高，而PI3K和AKT蛋白的表达降低。结论：桦木酮酸可能上调P53的活性，抑制PI3K和AKT蛋白，从而抑制细胞的存活通路促凋亡。

Abstract:Objective: To study the betulonic acid on the cell cycle and related protein expressions on mice of bearing H22 tumor cells. Method: Flow cytometray was used to observe the changes of betulonic acid on the cell cycle and P53 of H22 tumor cells. Immunohistochemistry was determined the espressions of PI3K and AKT. Result: Increasing the doses of betulonic acid, the number of H22 cells in S phase and G₂ phase was increasing gradually, it can speculate that when the betulonic acid act on cells, the cells were blocked in S and G₂ phase and inhibited the protein expressions of PI3K and AKT. Conclusion: Betulonic acid may be up-regulate the activity of P53 and inhibite the expressions of PI3K and AKT, so that it inhibited the survival pathway of tumor cells.

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