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期 刊 ：中国中药杂志 2006年 31卷 03期 页码：205.

关键词：白藜芦醇；壳聚糖；纳米粒；固化；

Keywords:resveratrol, chitosan, nanoparticles, solidification,

摘 要 ：目的：探索表面氨基游离的白藜芦醇壳聚糖纳米粒制备方法,以便连接配体实现主动靶向。方法:采用氯化钠沉淀法制备表面氨基游离的壳聚糖纳米粒;比较不同固化程度纳米溶液的浊度、体外释放、包封率、载药量和粒径等性质。结果:固化程度不同的纳米溶液经超声或水浴加热处理后,浊度降低值不同;含不同固化剂的纳米溶液均有明显缓释效果,随固化剂加入量增加,释放速度减慢;固化程度对包封率和载药量无明显影响。加固化剂200 μL的壳聚糖纳米粒粒径为487 nm,多分散指数为0.144。结论：制备的白藜芦醇壳聚糖纳米粒粒径相对较小,表面氨基游离,可用于主动靶向给药系统的设计。

Abstract:Objective: To prepare resveratrol chitosan nanoparticles with free amine groups on the surface so as to conjugate ligands,which will actively target to special tissues or organs.Method: The chitosan nanoparticles with free amine on the surface was prepared by sodium chloride precipitation.Nanoparticles with different solidification degrees were studied on turbidity,in vitro release, encapsulation efficiency,drug loading and diameter.Result: The turbidity of nanoparticles with various solidification degrees decreased at different rates after ultrasonic or water bath heating treatment.All nanoparticles mentioned above obviously shew sustained release.The rate of release was slowed down with the increase of solidification agents.Solidification had no obvious effects on the encapsulation efficiency and drug loading.The diameter of chitosan nanoparticles with 200 μL solidification agents was 487 nm.The polydispersion was 0.144.Conclusion: The diameter of the prepared nanoparticles was relatively small.The amine on the surface was free,which offered the possibility of designing the acive target drug delivery system.

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