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期 刊 ：中国中药杂志 2007年 32卷 11期 页码：1019.

关键词：杖；白藜芦醇；白藜芦醇苷；蒽醌；大孔吸附树脂；分离纯化；

Keywords:Polygonum cuspidatum, resveratrol, polydatin, anthraquinone, macroporous resin, separation and purification,

摘 要 ：目的：研究大孔吸附树脂纯化虎杖有效部位的工艺条件。方法：以白藜芦醇苷、白藜芦醇、大黄素、大黄素甲醚和总蒽醌的吸附量和解吸率为评价指标,筛选树脂,并优化了吸附和洗脱条件。结果：D101树脂对虎杖各有效成分具有较好的吸附分离性能,适于纯化虎杖有效部位。最佳工艺条件为：虎杖样品溶液1 BV,吸附流速2.4 BV·h^-1,上样后静置30 min,先用2 BV水洗涤,再用20%,50%,70%,95%乙醇各2,5,2,5 BV进行梯度洗脱,流速2 BV·h^-1,合并50%和95%乙醇洗脱液,即为纯化的总有效部位。结论：总有效成分纯度为36.87%,说明采用D101树脂分离纯化虎杖有效部位是可行的。

Abstract:Objective：To study the technological parameters of the purification process for effective part from Polygonum cuspidatum.Method：Using adsorption capacities and desorption rates of polydatin,resveratrol,emodin,physcion and total anthraquinone as the primary screening indexes,six resins were surveyed,and the optimized conditions of adsorption and desorption of the effective ingredients were studied.Result：Resin D101 gave good separation performance and was selected to purify the effective part in Polygonum cuspidatum.The optimum parameters were established as the following：1 BV (bed volume) sample extract was passed through the column with a flow rate of 2.4 BV·h^-1,30 min later,the column was washed with 2 BV water,2 BV 20% ethanol,5 BV 50% ethanol,2 BV 70% ethanol and 5 BV 95% ethanol,respectively.The combined 50% and 95% ethanolic elutes were concentrated to yield the purified effctive part.Conclusion：The purity of the total effective ingredients in the product was up to 36.87%. Macroporous resin D101 could be well used in separating and purifying the effective part from Polygonum cuspidatum.

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