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期 刊 ：中国中药杂志 2003年 28卷 07期 页码：647.

关键词：小白菊内酯；血管平滑肌细胞；细胞增殖；基因表达；

Keywords:parthenolide, vascular smooth muscle cell, cell proliferation, gene,

摘 要 ：目的:探讨小白菊内酯对血管平滑肌细胞增殖的影响及作用机制。方法:培养大鼠胸主动脉血管平滑肌细胞(VSMC),Western印迹杂交法检测c-fos,c-myc,p15,p16,p18,p19蛋白表达,流式细胞仪检测VSMC周期情况,[³H]TdR参入测定VSMC的DNA合成。结果:小白菊内酯以时间依赖关系抑制c-fos,c-myc蛋白表达,但不影响p15,p16,p18,p19蛋白表达,以剂量依赖关系使VSMC周期中的G₀/G₁期细胞比例明显增多,S期细胞比例显著减少,同时抑制VSMC的[³H]TdR参入。结论:小白菊内酯可能通过抑制c-fos,c-myc蛋白表达,而不是影响p15,p16,p18,p19蛋白表达来抑制VSMC增殖。

Abstract:Objective: To study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism. Method: Vascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [³H]-TdR incorporation. Result: Parthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn‘t affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G₀/G₁ phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [³H]-TdR incorporation in a dose dependent manner. Conclusion: Parthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.

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