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期 刊 ：中国中药杂志 2005年 30卷 11期 页码：812.

关键词：青天葵；组织培养；植株再生；

Keywords:Nervilia fordii, tissue culture, plant regeneration,

摘 要 ：目的:对青天葵进行组织培养并获得再生植株。方法:考察不同外植体、植物生长调节剂、添加物等对根状茎及再生植株生成的影响。结果:以球茎为外植体效果最好,6-BA2mg·L^-1诱导球茎生成根状茎的效果优于6-BA1mg·L^-1,椰汁、活性炭对根状茎的生长有促进作用。结论:青天葵球茎接种于1/2MS+6-BA2mg·L^-1培养基上,能够诱导出芽,芽接种于添加了10%椰汁和1‰活性炭的培养基中能够生成大量的根状茎,将白色的根状茎接种于1/2MS+1‰活性炭的培养基中能够先形成球茎,并进一步形成再生植株;将绿色的根状茎接种于1/2MS+6-BA2mg·L^-1+NAA2mg·L^-1的培养基上能够直接形成再生植株。

Abstract:Objective: Tissue culture of Nervilia fordii to get its regeneration. Method: Effects on indusement of rhizoma and plant regeneration of different implants, density of hormones, additives were studied. Result: The best implant was corm. Effect of 6-BA 2 mg·L^-1 were better than 6-BA 1 on rhizoma reducing. The coconut juice and active carbon could increase the growth of rhizoma. Conclusion: Bud could be induced on 1/2MS + 6-BA 2 mg·L^-1 by inoculating corm on culture mediem, and could grow lots of rhizoma after inoculating on the culture mediem containing 10% coconut juice and 1‰ active carbon. The white rhizoma could be induced to corms and regeneration plants on 1/2MS +1‰ active carbon.The green rhizoma could be induced directerly to regeneration plants on 1/2MS + 6-BA 2 + NAA 2.

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