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期 刊 ：中国中药杂志 2005年 30卷 13期 页码：986.

关键词：固相微萃取；气相色谱质谱法；菊花；挥发性成分；

Keywords:head space solid-phase microextraction, gas chromatography-mass spectrometry, Chrysanthemum morifolium, volatile component,

摘 要 ：目的:探讨固相微萃取-气相色谱-质谱技术提取分析贡黄菊和贡白菊挥发性成分的方法。方法:分别用不同温度、不同平衡时间、不同萃取纤维头,进行顶空固相微萃取操作,结合气相色谱-质谱联用技术对贡黄菊和贡白菊的挥发性成分进行鉴定,通过改变取样条件观察各成分峰面积的变化,并用面积归一法测定其相对含量。结果:在水浴75℃、平衡时间6h时,用100μm聚二甲基硅氧烷萃取纤维顶空提取贡黄菊和贡白菊的成分效果较好;共鉴定贡黄菊和贡白菊挥发性成分53种,其中共有成分35种。结论:顶空固相微萃取-气相色谱-质谱技术可以简便快捷准确地进行菊花挥发性成分的分析鉴定。

Abstract:Objective: To study a method for extraction and analysis of volatile components from Chrysanthemum morifolium ‘gonghuangjv‘ cv. nov(CM GHJ)and C. morifolium ‘gongbaijv‘ cv. nov (CM GBJ)by solid-phase microextraction (SPME) and gas chro matography-mass spectrometry(GC-MS). Method: The volatile components were extracted in different temperature, different balance period and different extraction fiber using head space solid-phase microextraction (HS-SPME), and were identified by GC-MS. The variety in integral area of each component was observed in different conditions and its relative content was determined by normalization of area. Result: The better condition of SPME for C. morifolium was that the sample was extracted using 100 μm polydimethylsiloxane (PDMS) extraction fiber after it had been balanced for 6 hours at 75℃. 53 Components from CM GHJ and CM GBJ were identified, and there were 35 same components in CM GHJ and CM GBJ. Conclusion: HS-SPME-GC-MS is convenient, rapid and reliable for analysis of volatile components in C. morifolium.

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