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期 刊 ：中国中药杂志 1999年 24卷 02期 页码：99.

关键词：地榆；没食子酸；高效液相色谱法；

Keywords:Garden Burnet, gallic acid, HPLC,

摘 要 ：目的：建立测定地榆中没食子酸含量的方法。方法：Ｓｈｉｍ-ｐａｃｋＣＬＣ-ＯＤＳ色谱柱，甲醇 ０．０２５ｍｏｌ／Ｌ磷酸（１５∶８５）为流动相，检测波长２７０ｎｍ。结果：没食子酸与其它组分的色谱峰达到基线分离，样品平均含量为０．２２％，加样回收率均值为９７．４％，ＲＳＤ为１．３％。结论：为控制中药地榆的内在质量提供了新的指标和方法。

Abstract:Objective:To establish a HPLC method for the determination of gallic acid in garden burnet.Method:The sample was extracted with methanol under ultrasonic management for 25 min.Chromatographic conditions:Shimadzu LC 6A with SPD 6AV detector at 270nm and auto injector SIL 6A,methanol 0.025 mol/L phosphoric acid(15∶85) as the mobile phase.Shin pack CLC ODS column(6.0mm×15mm).Result:Gallic acid in garden burnet was baseline separated and determined.The average content was 0.22%,recovery rate 97.4% and relative standard deviation 1.3%.Conclusion:Simple,rapid and accurate,the method provides new indexes for quality control of Garden Burnet.

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