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期 刊 ：中国中药杂志 1998年 23卷 11期 页码：651.

关键词：蟾酥；高效液相；华蟾酥毒基；脂蟾毒配基；

Keywords:HPLC:Venenum Bufonis, resibufogenin, cinobufagin,

摘 要 ：目的:建立HPLC法测定蟾酥药材中有效成分的含量。方法:选用0.5%磷酸二氢钾溶液 乙腈(50:50)(磷酸调节pH为3.25±0.02)为流动相测定蟾酥药材中华蟾酥毒基和脂蟾毒配基的含量。结果:测定组分具有良好的线性关系和分离度,华蟾酥毒基的平均回收率为100.35%,RSD=1.86%,脂蟾毒配基的平均回收率为100.38%,RSD=2.09%。结论:本方法简便、快速、准确,具有实用性

Abstract:Objective:To establish a HPLC method to determine the contents of active constituents in Venenum Bufonis.Method:In determining the contents of resibufogenin and cinobufagin in the traditional Chinese medicine Venenum Bufonis,the mobile phase was acetonitrile 0.5%KH2PO4 solution(50∶50)(pH adjusted with value phosphoric acid to 3.25±0.02).Result:The constituents thus determined have good linearity and separation.The average recovery of resibufogenin was 100.35%,RSD 1.86%;the average recovery of cinobufagin was 100.38%,RSD 2.09%.Conclusion:The method was convenient,rapid,accurate and practicable.

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