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期 刊 ：中国中药杂志 1998年 23卷 07期 页码：427.

关键词：双苄基异喹啉；阿霉素；长春新碱；抗癌作用；多药抗药性；

Keywords:bisbenzylisoquinoline, adriamycin, vincristine, anticancer activity, multidrug resistance,

摘 要 ：目的:研究双苄基异喹啉化合物Ys-96对阿霉素或长春新碱耐药株人癌细胞抗药性的逆转作用。方法:在体外培养的药物敏感株MCF-7及其阿霉素耐药株MCF-7/Ad以及药物敏感株KB及其长春新碱耐药株KBV200的体外试验系统中,采用MTT法研究耐药株人癌细胞在Ys-96作用下对阿霉素或长春新碱敏感性(IC50)的变化。结果:耐药株MCF-7/Ad和KBV200在Ys-96(1.00μmol/L)的作用下,阿霉素和长春新碱对其IC₅₀值分别接近于二药单独用药时对敏感株MCF-7和KB的IC₅₀。结论:Ys-96基本上能完全逆转MCF-7/Ad或KBV200对阿霉素或长春新碱的抗药性。

Abstract:Objective: Reversal effect of Ys-96,a bisbenzylisoquinoline,on the resistance of human cancer cells to adriamycin or vincristine was studied in vitroMethod:In an in vitro culture system of human cancer cells MCF-7 and its adriamycin resistant line MCF-7/Ad or KB and its vincristine resistant line KBV200,the sensitivity(IC₅₀)of the resistant cell lines to adriamycin or vincristine was evaluated with a MTT assay.Result:IC₅₀value of adriamycin or vincristine in combination with Ys 96 at a concentration of 1.00μmol/L against MCF-7/Ad or KBv200 was found to be close to that of adriamycin alone or vincristine alone against the sensitive cell line MCF-7 or KBConclusion:The drug resistance of MCF-7/Ad or KBv200 could be essentially reversed by Ys 96 at a concentration of 1.00μmol/L.

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