Abstract:The contents of oleanolic acid in the root of Achyranthes bidentata and A. aspera from different placesof production have been determined by dual-wavelengh TLC-scanning. The results show that the content of oleanolicacid is 0.91%~ 1.14 % in cultured A. bidentata, 0.78%~1.42 % in A. bidentata natural and 0.054% in A. aspera.The average recovery is 98.63% .