Toxicity-reducing and efficacy-maintaining function of fungal fermentative products in Tripterygium wilfordii by Ganoderma lucidum bi-direction solid fermentation
Abstract:Objective To survey the toxicity-reducing and efficacy-maintaining function of fungal fer-mentative products in Tripterygium wilfordii by Ganoderma lucidum bi-direction solid fermentation.Methods Bi-direction solid fermentation engineering was applied and Linglei fungal substance (G30)wasobtained, Its toxicity and pharmacodynamics were detected with regular acute toxicity test，immunologicaltest，and tests on autoimmune disease animal pattern. Results LD50 of G30 was higher than that of crude击ug. G30 had the inhibiting effect on proliferation of both T and B lymphocytes and could decrease theproduction of hemolysin in mice. G30 could decrease 24 h urine protein of nephritis model of rats caused byAdriamycin，and it had less toxicity on organs than T.wil fordii. G30 could alleviate foot swelling ofrheumatoid arthritis model. Conclusion Linglei fungal substance is obtained from the fermentative compo-sition of G. lucidum fungal and T. wilfordii, It shows low toxicity and effects of immunosuppression andalso has some therapeutic effects on the model of autoimmune diseases. The pharmocological effects sug-gest that the bi-directional fermentation engineering may possibly provide a new way to prepare the toxici-ty-reducing and efficacy-maintaining function of fungal fermentative products in T. wilfordii.