全 文 ：a靛玉红对照品;b青黛样品;1 靛玉红
图 2 青黛中靛玉红的高效液相样品色谱图
表 1 青黛中靛蓝、靛玉红含量测定结果 %
样品编号 产地 靛蓝含量 靛玉红含量
1 福建 0． 120 0． 095
2 福建 2． 209 0． 000
3 福建 0． 000 0． 000
4 福建 0． 087 0． 000
5 福建 2． 871 0． 043
6 福建仙游地区 1． 878 0． 064
7 福建 4． 574 0． 204
8 四川 3． 105 0． 015
9 安徽 3． 248 0． 085
10 福建 0． 082 0． 000
11 安徽 4． 574 0． 133
12 福建 2． 494 0． 000
13 福建 0． 000 0． 294
14 福建 4． 059 0． 000
在河北安国药材市场和安徽亳州药材市场进行调查发现，这
里的青黛商品主要产自福建仙游地区，商品分为甲级和乙级两
类，随机抽取了两批甲级样品，经检测发现，一批样品靛蓝含量合
格，靛玉红含量不合格;另一批样品靛蓝靛玉红含量均不合格。
所以青黛的质量问题十分严重，应引起有关部门的高度重视。
通过对青黛的主产地福建仙游地区进行实地调查发现，这里
生产的青黛以爵床科植物马蓝 Bap hicacanthus cusia(Nees)Bre-
mek．为原料。因福建省仙游县出产青黛的历史悠久、品质领先
于同类产品，故以专名建青黛驰名。建青黛的炮制加工方法
有传统和现代之分，传统的炮制方法沿袭了多年，方法粗糙原始，
一户一法，随意性大，含石灰等杂质较多，产量低，靛蓝靛玉红含
量较低，导致市场上的青黛商品多数达不到《中国药典》的规定。
基于上述结果，建议有关部门在加强药品质量监管的同时，
制定青黛炮制加工的规范，明确辅料用量、浸泡时间、加水量等参
数，在发扬传统加工工艺的基础上，运用现代精细加工技术，以产
业化代替作坊式生产，在提高青黛产量的同时，又可以保证其质
量，更好地满足临床用药的需求。
参考文献:
［1］ 国家药典委员会．中国药典，一部［S］．北京:中国医药科技出版社，
2010:185．
［2］ 杨步青，陈建斌，李新雄．建青黛及其原植物马蓝的研究进展［J］．
海峡药学，2008，20(12) :72．
［3］ 林夏楠，童剑斌，黄锦贵，等．建青黛加工炮制标准化研究［J］．海峡
药学，2009，21(3) :78．
［4］ 陈 郁，刘 舸．青黛炮制研究现状［J］．中国中药资讯，2010，2(15):255．
英语园地
Received date:2012-02-20; Revised date:2012-03-20
Fund:Medical science foundation of the administration of TCM of Guangdorg Province (Grant No． 101127)
Brief introduction of author:Wu Huifei (1977 －) ，female，assist professor at Zhognshan traditional medicine hospital of Chinese Traditional Medicine Universi-
ty of Guangzhou ，majoring at clinical pharmacology and TCM chemical research
* Corresponding author:Gao Youheng(1956 －) ，male，professor at insititute of nature product of Chinese Traditional Medicine University of Guangzhou，majo-
ring at research of TCM chemistry．
The Content of Chlorophytoside A in Chlorophytum laxum
WU Hui-fei1，MEI Quan-xi1，GAO You-heng2* ，WANG Yan3，ZHANG Dong-jing3
(1． Hospital of Traditional Chinese Medicine of Zhongshan，Zhongshan 528400，China;2． Depart-
ment of TCM Chemistry，Chinese Traditional Medical University of Guangzhou，Guangzhou 510405，
China;3． Department of Pharmacology，Guangdong Pharmacological University， Guangzhou
510405，China)
Abstract:Objective To establish an HPLC method for the determination of Chlorophytoside A in Chlorophytum
laxum R． Br．Methods A high performance liquid chromatography was used ． The measurement conditions were
as follows:TC － C18(Z)Agilent column (250mm × 4． 6mm，10μm) ;mobile phase:acetonitrile and 1% phos-
phoric acid(24:76) ;detection wavelength:200nm;column temperature:25℃;flow rate:1． 0 ml /min;injection:
10μl． Results Chlorophytoside A was separated well on the column from other components of the sample，the line-
ar range of chlorphenamine maleate was 0． 48μg ～ 4． 8μg，the standard curve of chlorphenamine maleate was as
follows:y = 1E + 06x － 28019，r2 = 0． 9999，the average recovery of Chlorophytoside A was 99． 02% with RSD 0．
81%(n = 6)． Conclusion The method is rapid，simple，accurate，reproducible and selective，which can be used
for the quality control of Chlorophytum laxum．
Key words:HPLC; Chlorophytum Laxum R． Br; Chlorophytoside; A determination of the content
DOI:doi:10． 3969 / j． issn． 1008-0805． 2012． 07． 093
Clc number:R284． 2 Document code:A Article ID:1008-0805(2012)07-1788-03
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Chlorophytum laxum R． Br is mainly distributed in south China．
The aerial part of the plant is used as a folk medicine for the treat-
ment of traumatic injury，poisonous snake bites，swelling and pain［1］．
As we had reported，［2 ～ 4］ in order to find out bioactive constituents，
from ethanolic extract of this plant we isolated eight compounds deter-
mined as Chlorophytoside A，hecogenin，Stigmastan，Daucosterol，4 ＇，
5，7 － trihydroxy － 6，8 － di － C － methyflavone，palmitric acid，β － si-
tosterol，respectively． Chlorophytoside A was a new Labdane Diterpene
Glycoside． To determine the quality of the Chlorophytum laxum R． Br，
we established an HPLC method for the determination of Chlorophyto-
side A in Chlorophytum laxum R． Br．
1 Instrumentation and materials
1． 1 Instrumentation HN1006 Ultrasonic washer(South China ultra-
sonic instrument Co．)、TianMei LC 2000HPLC、DIKMA ODS － C18
(4． 6 mm × 250 mm)、Sartourius /bp211b electronic balance、SHB －
IV double βvacuum pump with circulated water system、HWS26 elec-
tric － heated thermostatic waterbath、Chinese traditional medicine dis-
integrator．
1． 2 Plant material and standard The plant(whole plant)used for
the present study was collected from WuGui Mountain，Zhongshan cit-
y，Canton province． Plant materials were further identified as Chloro-
phytum laxum R． Br． by Li Wei，a professor from Chinese Traditional
Medicine University of Guangzhou． Standard sample was prepared by
ourselves(calibrated by HPLC，purity was above 98%)．
2 Method and Result
2． 1 Instrumentation and chromatographic conditions TC － C18(Z)
Agilent column (250 mm ×4． 6 mm，10μm) ;mobile phase:acetoni-
trile and 1% phosphoric acid (24:76) ;detection wavelength:
200nm;column temperature:25℃;flow rate:1． 0 ml /min;injection:
10μl． Theoretical plate number was above 3000 according to Chloro-
phytoside A．
Fig． 1 Standard solution of Chlorophytoside A
Fig． 2 Sample of Chlorophytum laxum R． Br．
2． 2． 1 Standard solution preparation A standard solution containing
Chlorophytoside A 6． 0mg was prepared by dissolving in 25ml methanol．
2． 2． 2 Sample preparation The powder samples(2． 0g)of leaves，
root，stem，whole plant were respectively extracted by methanol
(25ml)for 20 minutes，ultrasonic extraction，filtered，dried in water
bath，dissolved with methanol and transferred to a 25ml measuring
flask，and finally，obtained the volume to the calibration using metha-
nol and shaken evenly before filtering in a 0． 45μm membrance filter．
2． 3 Specification curve Injected 2． 0，5． 0，10． 0，15． 0，20． 0μl
standard solution into HPLC． Determined the Chlorophytoside A peak
area integral values according to the above chromatograph condition．
Chlorophytoside A standard curve:Y = 1E + 06x － 28019 (x was the
concentration of Chlorophytoside A and y was the peak area integral
value of Chlorophytoside A)． The Chlorophytoside A＇s linearity range
was 0． 48 ～ 4． 8μg，with a mean correlation coefficient r2 = 0． 999 9．
Table1 Standard solution chromatogram
content /μg peak area /y
0． 48 569289
1． 2 1 426 429
2． 4 2 953 471
3． 6 4 432 611
4． 8 5 949 487
2． 4 Precision Took Chlorophytum laxum leaves ＇ powder 2． 0g，ac-
cording to2． 2． 2 strip by the same method． And determined accord-
ing to the above chromatographic condition． Handled the specimen six
times，determined the chlorophytoside A peak area integral values．
RSD% was 1． 22% ． The results obtained confirmed a good precision
of the method developed．
2． 5 Stability Took the identical sample to supply the test solution
for 0，1，2，4，8，16 h，respectively，and determined the chlorophytoside
A peak area integral values． RSD% was 1． 69% ． The experimental
results indicated that samples were stable in 16 h．
2． 6 Reproducibility Took Chlorophytum laxum whole plant powder
2． 0g，according to2． 2． 2 strip by the same method． Determined the
chlorophytoside A peak area integral values according to the above
chromatograph condition，RSD% was 2． 48% ． The experiment results
indicated that the accuracy was good．
2． 7 Recovery Took 1． 000g Chlorophytum laxum whole plant pow-
der of the known content for each group and added 0． 85mg chloro-
phytoside A and calculate the spotting recovery rate． According to2．
2． 2 strip by the same method and computation content using stand-
ard curve method，the average recovery (n = 6)of chlorophytoside A
was 99． 02(RSD = 0． 81)． The results were shown in Tables 2．
Table2 Recovery rate of Chlorophytoside A
NO
Sampling
quantity
/g
Sample
content
/mg
Addition
/mg
Determination
/mg
Recovery
(%)
Average
(%)
RSD
(%)
1 1． 000 0． 84 0． 85 1． 67 98． 24
2 1． 000 0． 85 0． 85 1． 70 100
3 1． 000 0． 85 0． 85 1． 70 100 99． 02 0． 81
4 1． 000 0． 86 0． 85 1． 69 98． 83
5 1． 000 0． 87 0． 85 1． 69 98． 26
6 1． 000 0． 82 0． 85 1． 65 98． 80
The average recovery of chlorophytoside A was 99． 02% with
RSD0． 81%(n = 6)． The standard addition and recovery experiments
were conducted to determine the accuracy of the present method for
the quantification of chlorophytoside A in samples．
2． 8 Quantification of Chlorophytoside A in sample leaves Deter-
mined according to“2． 1”strip by the same method，injection was 10
μl，the HPLC chromatogram was shown in Figure 4，the determining
peak area was 2037621μV，calculated by standard curvilinear equa-
tions y = 1E + 0． 6x － 28019． The conclusion was obtained that every
10 μl contain Chlorophytoside A 1． 7 μg，which meant everylg leave
contained 0． 85 mg /g Chlorophytoside A。
Whole plant:determined according to2． 1strip by the same meth-
od，injection was 10 μl，the HPLC chromatogram was shown in Figure 5，
the determining peak area was 1172365 μV，calculated by standard curvi-
linear equations y =1E +0． 6x －28019． The conclusion was obtained that
every 10 μl contain chlorophytoside A 0． 986 μg，which meant every 1g
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LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH 2012 VOL． 23 NO． 7 时珍国医国药 2012 年第 23 卷第 7 期
whole plant contained 0． 493mg/g chlorophytoside A。
Fig． 3 HPLC chromatogram of Chlorophytoside
A in Chlorophytum laxum leaves
Fig． 4 HPLC chromatogram of Chlorophytoside
A in Chlorophytum laxum＇whole plant
Fig． 5 HPLC chromatogram of Chlorophytoside
A in Chlorophytum laxum＇root
Fig． 6 HPLC chromatogram of Chlorophytoside
A in Chlorophytum laxum＇stem
Table3 Content of Chlorophytoside
A in different parts of Chlorophytoside A
Medicinal parts Content of Chlorophytoside A
Leaves 0． 85mg /g
Whole plant 0． 493mg /g
Stem 0
Root 0
n = 3
3 Discussion
A sample and sensitive HPLC method is developed for the deter-
mination of chlorophytoside A in Chlorophytum laxum． This easy
method provides excellent sensitivity，accuracy and precision ，with
short retention time for Chlorophytoside A． We also found that the
stem and root don＇t have Chlorophytoside A，so we can determine that
the content of Chlorophytoside A to control the quality of Chlorophy-
tum laxum．
References:
［1］ Jiangsu New Medicinal college，Dictionary of Chinese Medicinal Materi-
als;Shanghai，Scientific and Technological Press;Shanghai，1977，vol．
1，62
［2］ HuiFeiWU，QuanXiMEI，YouHengGAO，ect． Chemical composition re-
search of Chlorophytum laxum［J］． Chinese medicinal plant，2004，27
(4) :259．
［3］ QuanXiMEI，YouHengGAO，HuiFeiWU，ect． Chemical composition re-
search of Chlorophytum laxumⅡ［J］． Chinese medicinal plant，2005，28
(6) :470．
［4］ You Heng Gao，Quan xi Mei，Wu Huifei． Chlorophytoside A，a New
Labdaen Diterpene Glycoside from Chlorophytum Laxum Chem． Bull．
Chinese Chemical Letters ，2005，16(7) :925 ～ 927．
收稿日期:2012-02-20; 修订日期:2012-03-30
基金项目:广东省中医药局立项科研课题(No． 101127)
作者简介:吴惠妃(1977-) ，女(汉族) ，广东中山人，现任中山市中医院副主任中药师，硕士学位，主要从事中药化学研究及临床药学工作．
* 通讯作者简介:高幼衡(1956-) ，男(汉族) ，江西南昌人，现任广州中医药大学教授，学士学位，主要从事中药化学研究工作．
三角草中三角草苷 A的含量测定
吴惠妃1，梅全喜1，高幼衡2* ，王 岩3，张东京3
(1．广州中医药大学附属中山医院，广东 中山 528400; 2．广州中医药大学 ，广东 广州 510006;
3．广东药学院，广东 广州 510006)
摘要:目的 建立三角草中三角草苷 A的含量测定方法。方法 高效液相色谱法测定三角草苷 A的含量，采用 Agilent TC
－ C18(Z)色谱柱 (250 mm ×4． 6 mm，10 μm) ，紫外检测波长为 200 nm，以乙腈 － 1%磷酸溶液(24∶ 76)为流动相，流速
为 1． 0 ml·min －1，柱温为 25℃，进样量 10μl，按外标法进行检测。结果 供试品中三角草苷 A得到较好的分离与测定，三
角草苷 A在 0． 48 ～ 4． 8 μg范围内线性关系良好，回归方程为:A = 1E + 06x － 28 019，r2 = 0． 999 9，平均回收率达 99． 02%，
RSD = 0． 81%(n = 6)。结论 所建立的方法快速，简便，准确，重复性好，专属性好，可用于评价三角草的质量。
关键词:高效液相色谱法; 三角草; 三角草苷 A; 含量测定
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时珍国医国药 2012 年第 23 卷第 7 期 LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH 2012 VOL． 23 NO． 7