The treatment of wheat ( Triticum aestivum L.) seedlings with CaCl2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca2+ chelator EGTA decreased the expression of the hsp26 gene. The expression of the wheat calmodulin gene CaM1-2 is rapidly up-regulated in wheat seedlings by heat shock at 37 ℃. The heat-induced up-regulation of CaM1-2 occurred earlier than that of the hsp26 gene did. Calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamid (W7) inhibited the expression of the hsp26 gene in wheat seedlings at 37 ℃ for 1 h. These results implied that the calcium-calmodulin is probably involved in the signal transduction of the hsp26 gene expression induced by heat shock.
钙-钙调素对小麦hsp26基因表达的影响
刘宏涛1,2 赵和1 李冰1,2 孙大业2 周人纲1*
（1. 河北省农林科学院农业物理生理生化研究所，石家庄050051；
2. 河北师范大学生命科学学院，石家庄050016）
关键词： 热激蛋白；钙’钙调素；基因表达；小麦
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